Overview

  • Product name

    Anti-SAE1 antibody [EPR15397(B)]
    See all SAE1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15397(B)] to SAE1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human SAE1 aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: Q9UBE0

  • Positive control

    • Hela, Jurkat, MCF7, 293, Raw264.7 and NIH 3T3 cell lysates; Hela and MCF7 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ICC/IF 1/100.

Target

  • Function

    The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
  • Tissue specificity

    Expression level increases during S phase and drops in G2 phase (at protein level).
  • Pathway

    Protein modification; protein sumoylation.
  • Sequence similarities

    Belongs to the ubiquitin-activating E1 family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activator of SUMO1 antibody
    • AOS1 antibody
    • HSPC140 antibody
    • Sae1 antibody
    • SAE1_HUMAN antibody
    • Sentrin/SUMO activating protein AOS1 antibody
    • SUA1 antibody
    • SUMO 1 activating enzyme E1 N subunit antibody
    • SUMO 1 activating enzyme subunit 1 antibody
    • SUMO-activating enzyme subunit 1 antibody
    • Ubiquitin like protein SUMO1 activating enzyme antibody
    • Ubiquitin-like 1-activating enzyme E1A antibody
    • UBL E1A antibody
    • UBLE1A antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling SAE1 with ab185949 at 1/100 dilution (red). Secondary antibody is a Goat anti-rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counterstain: DAPI (blue).

  • All lanes : Anti-SAE1 antibody [EPR15397(B)] (ab185949) at 1/1000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa



    Blocking buffer: 5% NFDM/TBST

  • Anti-SAE1 antibody [EPR15397(B)] (ab185949) at 1/10000 dilution + 293 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa



    Blocking buffer: 5% NFDM/TBST

  • All lanes : Anti-SAE1 antibody [EPR15397(B)] (ab185949) at 1/1000 dilution

    Lane 1 : Raw264.7 cell lysate
    Lane 2 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa

  • Immunofluorescent analysis of -20℃ acetone-fixed HeLa cells labeling SAE1 with ab185949 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counter stain: Dapi (blue).

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spinal cord)
Specification
Spinal cord
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: RT°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Jul 21 2016

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