Recombinant Anti-SAE1 antibody [EPR15397(B)] - BSA and Azide free (ab232618)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15397(B)] to SAE1 - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-SAE1 antibody [EPR15397(B)] - BSA and Azide free
See all SAE1 primary antibodies -
Description
Rabbit monoclonal [EPR15397(B)] to SAE1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: MCF7 cells.
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General notes
ab232618 is the carrier-free version of ab185949.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15397(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. -
Tissue specificity
Expression level increases during S phase and drops in G2 phase (at protein level). -
Pathway
Protein modification; protein sumoylation. -
Sequence similarities
Belongs to the ubiquitin-activating E1 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10055 Human
- Entrez Gene: 56459 Mouse
- Omim: 613294 Human
- SwissProt: Q9UBE0 Human
- SwissProt: Q9R1T2 Mouse
- Unigene: 515500 Human
- Unigene: 258530 Mouse
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Alternative names
- Activator of SUMO1 antibody
- AOS1 antibody
- HSPC140 antibody
see all
Images
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Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling SAE1 with ab185949 at 1/100 dilution (red). Secondary antibody is a Goat anti-rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counterstain: DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185949).
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Immunofluorescent analysis of -20? acetone-fixed HeLa cells labeling SAE1 with ab185949 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counter stain: Dapi (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185949).
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Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling SAE1 with ab185949 at 1/100 dilution (red). Secondary antibody is a Goat anti-rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counterstain: DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185949).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab232618 has not yet been referenced specifically in any publications.