Recombinant
RabMAb

Recombinant Anti-SAE1 antibody [EPR15397(B)] - BSA and Azide free (ab232618)

Overview

  • Product name

    Anti-SAE1 antibody [EPR15397(B)] - BSA and Azide free
    See all SAE1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15397(B)] to SAE1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human SAE1 aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: Q9UBE0

  • Positive control

    • ICC/IF: MCF7 cells.
  • General notes

    Ab232618 is the carrier-free version of ab185949. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232618 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
  • Tissue specificity

    Expression level increases during S phase and drops in G2 phase (at protein level).
  • Pathway

    Protein modification; protein sumoylation.
  • Sequence similarities

    Belongs to the ubiquitin-activating E1 family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activator of SUMO1 antibody
    • AOS1 antibody
    • HSPC140 antibody
    • Sae1 antibody
    • SAE1_HUMAN antibody
    • Sentrin/SUMO activating protein AOS1 antibody
    • SUA1 antibody
    • SUMO 1 activating enzyme E1 N subunit antibody
    • SUMO 1 activating enzyme subunit 1 antibody
    • SUMO-activating enzyme subunit 1 antibody
    • Ubiquitin like protein SUMO1 activating enzyme antibody
    • Ubiquitin-like 1-activating enzyme E1A antibody
    • UBL E1A antibody
    • UBLE1A antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling SAE1 with ab185949 at 1/100 dilution (red). Secondary antibody is a Goat anti-rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counterstain: DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185949).

  • Immunofluorescent analysis of -20? acetone-fixed HeLa cells labeling SAE1 with ab185949 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counter stain: Dapi (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185949).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling SAE1 with ab185949 at 1/100 dilution (red). Secondary antibody is a Goat anti-rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counterstain: DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185949).

References

ab232618 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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