Key features and details
- Rabbit monoclonal [EPR15398] to SAE1
- Suitable for: WB, ICC/IF, Flow Cyt, IP
- Reacts with: Rat, Human
Product nameAnti-SAE1 antibody [EPR15398]
See all SAE1 primary antibodies
DescriptionRabbit monoclonal [EPR15398] to SAE1
Tested applicationsSuitable for: WB, ICC/IF, Flow Cyt, IPmore details
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human SAE1 aa 200-300. The exact sequence is proprietary.
Database link: Q9UBE0
- Hela, Jurkat, 293 & MCF-7 cell lysates, Hela & MCF-7 cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab185552 in the following tested applications.
|WB||1/10000 - 1/50000. Predicted molecular weight: 38 kDa.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/50 - 1/70.|
FunctionThe heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Tissue specificityExpression level increases during S phase and drops in G2 phase (at protein level).
PathwayProtein modification; protein sumoylation.
Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Activator of SUMO1 antibody
- AOS1 antibody
- HSPC140 antibody
All lanes : Anti-SAE1 antibody [EPR15398] (ab185552) at 1/10000 dilution
Lane 1 : Hela cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293 cell lysate
Lane 4 : MCF-7 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa
Blocking buffer: 5% NFDM/TBST
Immunoprecipitation from 293 cells using ab185552 at 1/50 dilution. Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution. Blocking buffer: 5% NFDM/TBST.
Flow cytometry analysis of HeLa cells using ab185552 at 1/230 dilution. Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
Immunofluorescence analysis of MCF-7 cells labeling SAE1 with ab185552 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi.
Immunofluorescence analysis of HeLa cells labeling SAE1 with ab185552 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi.
ab185552 has been referenced in 2 publications.
- Yang Y et al. SAE1 promotes human glioma progression through activating AKT SUMOylation-mediated signaling pathways. Cell Commun Signal 17:82 (2019). PubMed: 31345225
- Wang F et al. Hypoxia regulates sumoylation pathways in intervertebral disc cells: implications for hypoxic adaptations. Osteoarthritis Cartilage 24:1113-24 (2016). PubMed: 26826302