Product nameAnti-SAE1 (phospho S185) antibody
See all SAE1 primary antibodies
DescriptionRabbit polyclonal to SAE1 (phospho S185)
Specificityab106096 shows minimal reactivity by ELISA against the non-phosphorylated form of the immunising peptide
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Chimpanzee
A synthetic peptide corresponding to a region surrounding serine 185 of Human SAE1.
- HeLa cells treated with phosphatase inhibitors.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab106096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 38 kDa.|
|ELISA||1/5000 - 1/25000.|
FunctionThe heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Tissue specificityExpression level increases during S phase and drops in G2 phase (at protein level).
PathwayProtein modification; protein sumoylation.
Sequence similaritiesBelongs to the ubiquitin-activating E1 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Activator of SUMO1 antibody
- AOS1 antibody
- HSPC140 antibody
All lanes : Anti-SAE1 (phospho S185) antibody (ab106096) at 1/1000 dilution
Lane 1 : HeLa whole cell protein
Lane 2 : HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail
Lysates/proteins at 20 µg per lane.
All lanes : HRP-conjugated secondary
Developed using the ECL technique.
Predicted band size: 38 kDa
10% SDS-PAGE. Lower panel shows tubulin loading control
ab106096 has not yet been referenced specifically in any publications.