Anti-SAE2 / UBA2 antibody (ab241611)
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Overview
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Product name
Anti-SAE2 / UBA2 antibody
See all SAE2 / UBA2 primary antibodies -
Description
Rabbit polyclonal to SAE2 / UBA2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human SAE2/ UBA2 aa 590-640. The exact sequence is proprietary. (NP_005490.1)
Database link: Q9UBT2 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 cell lysate. IP: HeLa cell lysate. IHC-P: Human prostate carcinoma and mouse renal cell carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab241611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. -
Pathway
Protein modification; protein sumoylation. -
Sequence similarities
Belongs to the ubiquitin-activating E1 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10054 Human
- Entrez Gene: 50995 Mouse
- Omim: 613295 Human
- SwissProt: Q9UBT2 Human
- SwissProt: Q9Z1F9 Mouse
- Unigene: 631580 Human
- Unigene: 27560 Mouse
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Alternative names
- Anthracycline associated resistance ARX antibody
- Anthracycline-associated resistance ARX antibody
- ARX antibody
see all
Images
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Western blot - Anti-SAE2 / UBA2 antibody (ab241611)All lanes : Anti-SAE2 / UBA2 antibody (ab241611) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 3 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAE2 / UBA2 antibody (ab241611)Formalin-fixed, paraffin-embedded human prostate carcinoma (left) and mouse renal cell carcinoma (right) stained for SAE2 / UBA2 using ab241611 at 1/200 dilution in immunohistochemical analysis. DAB staining.
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Immunoprecipitation - Anti-SAE2 / UBA2 antibody (ab241611)SAE2 / UBA2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241611 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab241611 at 0.4 µg/ml.
Lane 1: ab241611 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 1 second.
Datasheets and documents
References
ab241611 has not yet been referenced specifically in any publications.
