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Rabbit polyclonal to SAE2 / UBA2
Predicted to work with:
Mouse, Rat, Chicken, Cow, Dog, Zebrafish
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human SAE2/ UBA2.
(Peptide available as
This antibody gave a positive signal in both Human brain and Human heart tissue lysates aswell as the following whole cell lysates: HeLa; Jurkat; HepG2; HEK293; Caco2.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Protein modification; protein sumoylation.
Belongs to the ubiquitin-activating E1 family.
Information by UniProt
Anthracycline associated resistance ARX antibody
Anthracycline-associated resistance ARX antibody
Western blot - Anti-SAE2 / UBA2 antibody (ab95835)
All lanes :
Anti-SAE2 / UBA2 antibody (ab95835) at 1 µg/ml
Lane 1 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 :
HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 :
HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 :
Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lane 6 :
Human brain tissue lysate - total protein (
Lane 7 :
Human heart tissue lysate - total protein (
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
Abcam recommends using milk as the blocking agent at a concentration of 3%. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"