Recombinant
RabMAb

Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955)

Overview

  • Product name
    Anti-SAE2 / UBA2 antibody [EPR14880]
    See all SAE2 / UBA2 primary antibodies
  • Description
    Rabbit monoclonal [EPR14880] to SAE2 / UBA2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IP, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SAE2/ UBA2 aa 50-150. The exact sequence is proprietary.
    Database link: Q9UBT2

  • Positive control
    • HeLa, A549, K562, Raw264.7 and PC-12 cell lysates; Human colon tissue; Mouse brain tissue; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14880
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab185955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.
IP 1/30 - 1/50.
ICC/IF 1/100.
WB 1/1000 - 1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 71 kDa).

Target

  • Function
    The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
  • Pathway
    Protein modification; protein sumoylation.
  • Sequence similarities
    Belongs to the ubiquitin-activating E1 family.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Anthracycline associated resistance ARX antibody
    • Anthracycline-associated resistance ARX antibody
    • ARX antibody
    • FLJ13058 antibody
    • HRIHFB2115 antibody
    • SAE 2 antibody
    • SAE2 antibody
    • SAE2_HUMAN antibody
    • SUMO 1 activating enzyme subunit 2 antibody
    • SUMO activating enzyme subunit 2 antibody
    • SUMO-activating enzyme subunit 2 antibody
    • UBA2 antibody
    • UBA2 ubiquitin activating enzyme E1 homolog antibody
    • Ubiquitin like 1 activating enzyme E1B antibody
    • Ubiquitin like modifier activating enzyme 2 antibody
    • Ubiquitin-like 1-activating enzyme E1B antibody
    • UBLE1B antibody
    see all

Images

  • All lanes : Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 71 kDa
    Observed band size: 90 kDa (why is the actual band size different from the predicted?)

  • All lanes : Anti-SAE2 / UBA2 antibody [EPR14880] (ab185955) at 1/1000 dilution

    Lane 1 : Raw264.7 cell lysate
    Lane 2 : PC-12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 71 kDa
    Observed band size: 90 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SAE2 / UBA2 with ab185955 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling SAE2 / UBA2 with ab185955 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunofluorescent analysis of acetone-fixed HeLa cells labeling SAE2 / UBA2 with ab185955 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.

  • Western blot analysis of SAE2 / UBA2 in K562 cell lysate immunoprecipitated with ab185955 at 1/50 dilution.

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

References

ab185955 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (293T cells)
Gel Running Conditions
Reduced Denaturing (3-8% gel)
Loading amount
5 µg
Specification
293T cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Oct 18 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
50 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 01 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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