Overview

  • Product name

  • Description

    Rabbit polyclonal to SAF-B2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human SAF-B2 aa 351-502.
    Database link: AAH25279

  • Positive control

    • 293T, A431 and Jurkat cell lines. HBL435 xenograft.
  • General notes

    Previously labelled as Scaffold attachment factor B2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab104220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 107 kDa.
IHC-P 1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer ( PH8.0).
ICC/IF 1/100 - 1/200.

Target

  • Function

    Binds to scaffold/matrix attachment region (S/MAR) DNA. Can function as an estrogen receptor corepressor and can also inhibit cell proliferation.
  • Tissue specificity

    Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 SAP domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DKFZP779C1727 antibody
    • HAP antibody
    • HET antibody
    • hsp27 ere tata binding antibody
    • KIAA0138 antibody
    • SAF B antibody
    • SAF B2 antibody
    • SAF-B2 antibody
    • SAFB antibody
    • SAFB1 antibody
    • SAFB2 antibody
    • SAFB2_HUMAN antibody
    • Scaffold attachment factor B2 antibody
    see all

Images

  • Anti-SAF-B2 antibody (ab104220) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 107 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed A431, using ab104220 at 1/200 dilution. Lower picture costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using ab104220 at 1/100 dilution.

References

ab104220 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab104220.
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