Key features and details
- Rabbit polyclonal to SAF-B2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SAF-B2 antibody
See all SAF-B2 primary antibodies
DescriptionRabbit polyclonal to SAF-B2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human SAF-B2 aa 351-502.
Database link: AAH25279
- 293T, A431 and Jurkat cell lines. HBL435 xenograft.
Previously labelled as Scaffold attachment factor B2.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
Our Abpromise guarantee covers the use of ab104220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 107 kDa.|
|IHC-P||1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer ( PH8.0).|
|ICC/IF||1/100 - 1/200.|
FunctionBinds to scaffold/matrix attachment region (S/MAR) DNA. Can function as an estrogen receptor corepressor and can also inhibit cell proliferation.
Tissue specificityExpressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
Contains 1 SAP domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- DKFZP779C1727 antibody
- HAP antibody
- HET antibody
Anti-SAF-B2 antibody (ab104220) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 107 kDa
7.5% SDS PAGE
Immunofluorescence analysis of methanol-fixed A431, using ab104220 at 1/200 dilution. Lower picture costained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using ab104220 at 1/100 dilution.
ab104220 has not yet been referenced specifically in any publications.