Key features and details
- Cell-permeable, selective, potent Smoothened agonist
- CAS Number: 912545-86-9
- Purity: > 98%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Product nameSAG (Smo agonist), Smoothened agonist
DescriptionCell-permeable, selective, potent Smoothened agonist
Cell-permeable, selective, potent Smoothened agonist (EC50 = 3 nM). Binds the Smo heptahelical bundle that antagonizes cyclopamine (ab120392) action. Active in vitro and in vivo.
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab142160 has been referenced in 5 publications.
- Tessier S et al. NFAT5/TonEBP controls early acquisition of notochord phenotypic markers, collagen composition, and sonic hedgehog signaling during mouse intervertebral disc embryogenesis. Dev Biol 455:369-381 (2019). PubMed: 31301300
- Kim BH et al. Deficiency of calpain-6 inhibits primary ciliogenesis. BMB Rep 52:619-624 (2019). PubMed: 31619317
- Coulter ME et al. The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles. Cell Rep 24:973-986.e8 (2018). PubMed: 30044992
- Meerang M et al. Antagonizing the Hedgehog Pathway with Vismodegib Impairs Malignant Pleural Mesothelioma Growth In Vivo by Affecting Stroma. Mol Cancer Ther 15:1095-105 (2016). PubMed: 26839306
- Xing Y et al. Liver X receptor a is essential for the capillarization of liver sinusoidal endothelial cells in liver injury. Sci Rep 6:21309 (2016). PubMed: 26887957