Anti-SAGE1 antibody (ab121160)
Key features and details
- Rabbit polyclonal to SAGE1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SAGE1 antibody
See all SAGE1 primary antibodies -
Description
Rabbit polyclonal to SAGE1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence, corresponding to amino acids 247-387 of Human SAGE1.
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Positive control
- Human spleen tissue; Human U 2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121160 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Tissue specificity
Expressed mainly in bladder, lung, head and neck carcinomas. Not expressed in normal tissues except for testis. - Information by UniProt
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Database links
- Entrez Gene: 55511 Human
- SwissProt: Q9NXZ1 Human
- Unigene: 195292 Human
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Alternative names
- Cancer/testis antigen 14 antibody
- CT14 antibody
- RP11-97N5.1 antibody
see all
Images
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ab121160 at 1/350 dilution staining SAGE1 in Paraffin-embedded Human Spleen tissue by Immunohistochemistry.
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ab121160 at 4 µg/ml staining SAGE1 in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).
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Immunohistochemical staining of Human testis shows strong nuclear positivity in cells in seminiferus ducts using ab121160
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121160 has not yet been referenced specifically in any publications.