Key features and details
- Rabbit polyclonal to SAHH
- Suitable for: WB, ELISA
- Reacts with: Recombinant fragment
- Isotype: IgG
Product nameAnti-SAHH antibody
See all SAHH primary antibodies
DescriptionRabbit polyclonal to SAHH
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Mouse, Rat, Human
Synthetic peptide corresponding to Human SAHH (N terminal).
This product was previously labelled as S adenosylhomocysteine hydrolase
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab56146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.|
|ELISA||Use at an assay dependent dilution.|
FunctionAdenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
PathwayAmino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.
Involvement in diseaseDefects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD) [MIM:613752]. A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy.
Sequence similaritiesBelongs to the adenosylhomocysteinase family.
Cellular localizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- Adenosylhomocysteinase antibody
- AdoHcyase antibody
- ahcY antibody
All lanes : Anti-SAHH antibody (ab56146) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
ab56146 has been referenced in 1 publication.
- Hine C et al. Hypothalamic-Pituitary Axis Regulates Hydrogen Sulfide Production. Cell Metab 25:1320-1333.e5 (2017). PubMed: 28591635