Recombinant Anti-SAHH antibody [EPR9260] (ab151734)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-SAHH antibody [EPR9260]
See all SAHH primary antibodies -
Description
Rabbit monoclonal [EPR9260] to SAHH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human SAHH aa 1-100. The exact sequence is proprietary.
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Positive control
- HeLa, A549, HepG2, and Jurkat whole cell lysate (ab7899).
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as S adenosylhomocysteine hydrolase
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9260 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab151734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 48 kDa. | |
| IP | 1/10 - 1/100. |
Target
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Function
Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. -
Pathway
Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. -
Involvement in disease
Defects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD) [MIM:613752]. A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy. -
Sequence similarities
Belongs to the adenosylhomocysteinase family. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 191 Human
- Omim: 180960 Human
- SwissProt: P23526 Human
- Unigene: 388004 Human
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Alternative names
- Adenosylhomocysteinase antibody
- AdoHcyase antibody
- ahcY antibody
see all
Images
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![Western blot - Anti-SAHH antibody [EPR9260] (ab151734)](https://www.abcam.com/ps/products/151/ab151734/Images/ab151734-158816-anti-s-adenosylhomocysteine-hydrolase-antibody-epr9260-western-blot.jpg)
Western blot - Anti-SAHH antibody [EPR9260] (ab151734)All lanes : Anti-SAHH antibody [EPR9260] (ab151734) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 48 kDa
Datasheets and documents
References
This product has been referenced in:
- Lee HO et al. S-adenosylhomocysteine hydrolase over-expression does not alter S-adenosylmethionine or S-adenosylhomocysteine levels in CBS deficient mice. Mol Genet Metab Rep 15:15-21 (2018). Read more (PubMed: 30023284) »
