Recombinant Anti-SAHH antibody [EPR9260] (ab151734)
![Western blot - Anti-SAHH antibody [EPR9260] (ab151734)](https://www.abcam.com/ps/products/151/ab151734/Images/ab151734-158816-anti-s-adenosylhomocysteine-hydrolase-antibody-epr9260-western-blot.jpg "Western blot - Anti-SAHH antibody [EPR9260] (ab151734)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9260] to SAHH
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-SAHH antibody [EPR9260]
See all SAHH primary antibodies -
Description
Rabbit monoclonal [EPR9260] to SAHH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human SAHH aa 1-100. The exact sequence is proprietary.
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Positive control
- HeLa, A549, HepG2, and Jurkat whole cell lysate (ab7899).
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as S adenosylhomocysteine hydrolase
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9260 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab151734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 48 kDa. | |
| IP | 1/10 - 1/100. |
Target
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Function
Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. -
Pathway
Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. -
Involvement in disease
Defects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD) [MIM:613752]. A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy. -
Sequence similarities
Belongs to the adenosylhomocysteinase family. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 191 Human
- Omim: 180960 Human
- SwissProt: P23526 Human
- Unigene: 388004 Human
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Alternative names
- Adenosylhomocysteinase antibody
- AdoHcyase antibody
- ahcY antibody
see all
Images
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Western blot - Anti-SAHH antibody [EPR9260] (ab151734)All lanes : Anti-SAHH antibody [EPR9260] (ab151734) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 48 kDa -
![Anti-SAHH antibody [EPR9260] (ab151734)](https://www.abcam.com/ps/products/151/ab151734/Images/ab151734-2-benefits-of-recombinant-antibodies.png)
Anti-SAHH antibody [EPR9260] (ab151734)
Protocols
Datasheets and documents
References (1)
ab151734 has been referenced in 1 publication.
- Lee HO et al. S-adenosylhomocysteine hydrolase over-expression does not alter S-adenosylmethionine or S-adenosylhomocysteine levels in CBS deficient mice. Mol Genet Metab Rep 15:15-21 (2018). PubMed: 30023284
