RecombinantRabMAb

Recombinant Anti-SAHH antibody [EPR9261] (ab134966)

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Western blot - Anti-SAHH antibody [EPR9261] (ab134966)
  • Anti-SAHH antibody [EPR9261] (ab134966)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9261] to SAHH
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-SAHH antibody [EPR9261]
    See all SAHH primary antibodies

  • Description

    Rabbit monoclonal [EPR9261] to SAHH

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human SAHH aa 350-450. The exact sequence is proprietary.

  • Positive control

    • HeLa, A549, HepG2, and Jurkat whole cell lysate (ab7899).

  • General notes

     

     

     This product was previously labelled as S adenosylhomocysteine hydrolase

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • Target

    • Function

      Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.

    • Pathway

      Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.

    • Involvement in disease

      Defects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD) [MIM:613752]. A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy.

    • Sequence similarities

      Belongs to the adenosylhomocysteinase family.

    • Cellular localization

      Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Adenosylhomocysteinase antibody
      • AdoHcyase antibody
      • ahcY antibody
      • S adenosyl L homocysteine hydrolase antibody
      • S adenosylhomocysteine hydrolase antibody
      • S-adenosyl-L-homocysteine hydrolase antibody
      • SAHH antibody
      • SAHH_HUMAN antibody
      see all

    Images

    • Western blot - Anti-SAHH antibody [EPR9261] (ab134966)
      Western blot - Anti-SAHH antibody [EPR9261] (ab134966)
      All lanes : Anti-SAHH antibody [EPR9261] (ab134966) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 47 kDa

    • Anti-SAHH antibody [EPR9261] (ab134966)
      Anti-SAHH antibody [EPR9261] (ab134966)

    References (2)

    Publishing research using ab134966? Please let us know so that we can cite the reference in this datasheet.

    ab134966 has been referenced in 2 publications.

    • Belužic L  et al. Knock-down of AHCY and depletion of adenosine induces DNA damage and cell cycle arrest. Sci Rep 8:14012 (2018). PubMed: 30228286
    • Park SJ  et al. Inhibition of S-adenosylhomocysteine hydrolase decreases cell mobility and cell proliferation through cell cycle arrest. Am J Cancer Res 5:2127-38 (2015). PubMed: 26328244

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