Recombinant Anti-SAHH antibody [EPR9261] (ab134966)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-SAHH antibody [EPR9261]
See all SAHH primary antibodies -
Description
Rabbit monoclonal [EPR9261] to SAHH -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human SAHH aa 350-450. The exact sequence is proprietary.
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Positive control
- HeLa, A549, HepG2, and Jurkat whole cell lysate (ab7899).
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General notes
This product was previously labelled as S adenosylhomocysteine hydrolase
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9261 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab134966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 47 kDa. |
Target
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Function
Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. -
Pathway
Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. -
Involvement in disease
Defects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD) [MIM:613752]. A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy. -
Sequence similarities
Belongs to the adenosylhomocysteinase family. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 191 Human
- Entrez Gene: 269378 Mouse
- Entrez Gene: 29443 Rat
- Omim: 180960 Human
- SwissProt: P23526 Human
- SwissProt: P50247 Mouse
- SwissProt: P10760 Rat
- Unigene: 388004 Human
see all -
Alternative names
- Adenosylhomocysteinase antibody
- AdoHcyase antibody
- ahcY antibody
see all
Images
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![Western blot - Anti-SAHH antibody [EPR9261] (ab134966)](https://www.abcam.com/ps/products/134/ab134966/Images/ab134966-149207-anti-s-adenosylhomocysteine-hydrolase-antibody-epr9261-western-blot.jpg)
Western blot - Anti-SAHH antibody [EPR9261] (ab134966)All lanes : Anti-SAHH antibody [EPR9261] (ab134966) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 47 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Belužic L et al. Knock-down of AHCY and depletion of adenosine induces DNA damage and cell cycle arrest. Sci Rep 8:14012 (2018). Read more (PubMed: 30228286) »
