Overview

  • Product name

    Anti-Salivary alpha amylase antibody
    See all Salivary alpha amylase primary antibodies
  • Description

    Rabbit polyclonal to Salivary alpha amylase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Salivary alpha amylase (N terminal).

  • Positive control

    • IHC-P: Rat spleen tissue. WB: Rat and mouse pancreas tissue lysate, human PANC-1 whole cell lysates. Rat liver tissue lysate, Rat small intestine tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab125230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 57 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Sequence similarities

    Belongs to the glycosyl hydrolase 13 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • amylase, salivary, B antibody
    • 1 4 alpha D glucan glucanohydrolase antibody
    • 1 antibody
    • 1,4 alpha D glucan glucanohydrolase 2B antibody
    • 1,4-alpha-D-glucan glucanohydrolase 1 antibody
    • 4-alpha-D-glucan glucanohydrolase 1 antibody
    • Alpha amylase antibody
    • Alpha-amylase 1 antibody
    • AMY1 antibody
    • AMY1_HUMAN antibody
    • AMY1C antibody
    • AMY2 antibody
    • AMY2B antibody
    • AMY3 antibody
    • amylase, alpha 1A (salivary) antibody
    • Amylase, alpha 1B (salivary) antibody
    • amylase, alpha 1C (salivary) antibody
    • amylase, salivary, alpha-1A antibody
    • amylase, salivary, alpha-1B antibody
    • amylase, salivary, alpha-1C antibody
    • amylase, salivary, C antibody
    • glycogenase antibody
    • HXA antibody
    • PA antibody
    • Salivary alpha-amylase antibody
    • salivary amylase alpha 1A antibody
    • salivary amylase alpha 1B antibody
    • salivary amylase alpha 1C antibody
    see all

Images

  • All lanes : Anti-Salivary alpha amylase antibody (ab125230) at 0.5 µg/ml

    Lane 1 : human PANC-1 whole cell lysates
    Lane 2 : rat pancreas tissue lysates
    Lane 3 : mouse pancreas tissue lysates

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG-HRP at 1/10000 dilution

    Predicted band size: 57 kDa

  • All lanes : Anti-Salivary alpha amylase antibody (ab125230) at 1 µg/ml

    Lane 1 : Rat liver tissue lysate
    Lane 2 : Rat small intestine tissue lysate

    Predicted band size: 57 kDa

  • ab125230 at 1µg/ml staining Alpha Amylase in paraffin embedded Rat Spleen Tissue by immunohistochemistry.

References

ab125230 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.


To answer the first of the customer's question: yes, both antibodies should bind against the active, native protein.


The difference between the two methods/ the two purification gradesis the following:


Affinity purification with immobilized antigen: Covalentlyimmobilizing purified antigen (i.e., the peptide or hapten used as the immunogen to induce production of antibody by the host animal) to an affinity support allows the specific antibody to be purified from crude samples.

The isolation of the immunoglobulin G (IgG) fraction (containing antibodies of all specificities) from a complex protein mixture such as antiserum, ascites fluid, or hybridoma supernatant. The basic procedure utilizes saturated ammonium sulfate solution to precipitate the IgG fraction, while an alternate protocol describes fractionation of IgG by chromatography.

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