The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/5000.
In a simple indirect ELISA with 400 ng of human salivary amylase bound to the plate, the 50% OD was observed at an antibody dilution of 1/5K. Under the same conditions, the end point was observed at 1/500K.
1/50 - 1/200.
Use at an assay dependent concentration. Predicted molecular weight: 58 kDa. PubMed: 24056972