Recombinant
RabMAb

Recombinant Anti-Salivary alpha amylase antibody [EPR19605] (ab201450)

Overview

  • Product name

    Anti-Salivary alpha amylase antibody [EPR19605]
    See all Salivary alpha amylase primary antibodies
  • Description

    Rabbit monoclonal [EPR19605] to Salivary alpha amylase
  • Host species

    Rabbit
  • Specificity

    This product reacts with Salivary alpha amylase (P04745), Pancreatic alpha amylase (P04746) and Carcinoid alpha amylase (P19961) due to high sequence homology.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Salivary alpha amylase aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: P04745

  • Positive control

    • WB: Human salivary alpha-amylase fragment recombinant protein; human pancreatic alpha-amylase fragment recombinant protein; human alpha-carcinoid alpha-amylase fragment recombinant protein; human parotid gland and pancreas lysates. IHC-P: Human parotid gland and pancreas tissues
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab201450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 58 kDa).

Target

  • Sequence similarities

    Belongs to the glycosyl hydrolase 13 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • amylase, salivary, B antibody
    • 1 4 alpha D glucan glucanohydrolase antibody
    • 1 antibody
    • 1,4 alpha D glucan glucanohydrolase 2B antibody
    • 1,4-alpha-D-glucan glucanohydrolase 1 antibody
    • 4-alpha-D-glucan glucanohydrolase 1 antibody
    • Alpha amylase antibody
    • Alpha-amylase 1 antibody
    • AMY1 antibody
    • AMY1_HUMAN antibody
    • AMY1C antibody
    • AMY2 antibody
    • AMY2B antibody
    • AMY3 antibody
    • amylase, alpha 1A (salivary) antibody
    • Amylase, alpha 1B (salivary) antibody
    • amylase, alpha 1C (salivary) antibody
    • amylase, salivary, alpha-1A antibody
    • amylase, salivary, alpha-1B antibody
    • amylase, salivary, alpha-1C antibody
    • amylase, salivary, C antibody
    • glycogenase antibody
    • HXA antibody
    • PA antibody
    • Salivary alpha-amylase antibody
    • salivary amylase alpha 1A antibody
    • salivary amylase alpha 1B antibody
    • salivary amylase alpha 1C antibody
    see all

Images

  • All lanes : Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/10000 dilution

    Lane 1 : Human salivary alpha-amylase fragment recombinant protein
    Lane 2 : Human pancreatic alpha-amylase fragment recombinant protein
    Lane 3 : Human alpha-carcinoid alpha-amylase fragment recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 58 kDa
    Observed band size: 22,22,50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human salivary alpha-amylase contains aa311-511 with a GST-tag.  Human pancreatic alpha-amylase and alpha-carcinoid alpha-amylase fragment recombinant proteins contain aa311-511 with a His-Tag®.  These three recombinant proteins were made in-house.

  • Lanes 1-3 : Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/20000 dilution
    Lanes 4-6 : Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/1000 dilution

    Lane 1 : Human parotid gland lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : Human fetal heart lysate
    Lane 5 : Human fetal kidney lysate
    Lane 6 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 58 kDa
    Observed band size: 57 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile  observed is consistent with what has been described in the literature (PMID: 3261213).

  • Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/5000 dilution + Human pancreas lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 58 kDa
    Observed band size: 57 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded human parotid gland tissue labeling salivary alpha-amylase + pancreatic alpha-amylase + alpha-carcinoid alpha-amylase with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human parotid gland is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling salivary alpha-amylase + pancreatic alpha-amylase + alpha-carcinoid alpha-amylase with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human pancreas is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: No staining on human colon.

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab201450 has not yet been referenced specifically in any publications.

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