SALL2 overexpression 293T lysate (whole cell) (ab94229)
Overview
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Product name
SALL2 overexpression 293T lysate (whole cell) -
General notes
ab94229 is a 293T cell transfected lysate in which Human SALL2 has been transiently over-expressed using a pCMV-SALL2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
The region-specific homeotic gene sal (spalt) of Drosophila encodes a zinc finger protein of unusual but characteristic structure. These unique features were used to isolate sal-like genes from humans. Two sal-like transcription units SALL1 and SALL2, located on chromosomes 16q12.1 and 14q11.1-q12.1 respectively, have been isolated and characterized. SALL1 and SALL2 transcripts are expressed in a limited number of adult organs, including the brain. SALL2 is evenly expressed in different brain areas. Transcripts of both genes can be detected in fetal brain neurons. The arrangement of sal-like zinc finger domains and their high degree of sequence similarity suggest a novel and conserved subfamily of human zinc finger transcription factors that is closely related to the Drosophila gene product encoded by the gene sal.
Applications
Our Abpromise guarantee covers the use of ab94229 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent dilution. |
Images
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SDS-PAGE - SALL2 overexpression 293T lysate (whole cell) (ab94229)ab94229 at 15μg/lane on an SDS-PAGE gel -
Western blot - SALL2 overexpression 293T lysate (whole cell) (ab94229)All lanes : Anti-SALL2 antibody (ab55723) at 1/500 dilution
Lane 1 :SALL2 overexpression 293T lysate (whole cell) (ab94229)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
References (0)
ab94229 has not yet been referenced specifically in any publications.
