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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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SALL2 overexpression 293T lysate (whole cell) (ab94229)

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  • SDS
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SDS-PAGE - SALL2 overexpression 293T lysate (whole cell) (ab94229)
  • Western blot - SALL2 overexpression 293T lysate (whole cell) (ab94229)

Overview

  • Product name

    SALL2 overexpression 293T lysate (whole cell)
  • General notes

    ab94229 is a 293T cell transfected lysate in which Human SALL2 has been transiently over-expressed using a pCMV-SALL2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes

  • Tested applications

    Suitable for: WBmore details

Properties

  • Mycoplasma free

    Yes
  • Form

    Liquid
  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
  • Background

    The region-specific homeotic gene sal (spalt) of Drosophila encodes a zinc finger protein of unusual but characteristic structure. These unique features were used to isolate sal-like genes from humans. Two sal-like transcription units SALL1 and SALL2, located on chromosomes 16q12.1 and 14q11.1-q12.1 respectively, have been isolated and characterized. SALL1 and SALL2 transcripts are expressed in a limited number of adult organs, including the brain. SALL2 is evenly expressed in different brain areas. Transcripts of both genes can be detected in fetal brain neurons. The arrangement of sal-like zinc finger domains and their high degree of sequence similarity suggest a novel and conserved subfamily of human zinc finger transcription factors that is closely related to the Drosophila gene product encoded by the gene sal.

Applications

Our Abpromise guarantee covers the use of ab94229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • SDS-PAGE - SALL2 overexpression 293T lysate (whole cell) (ab94229)
    SDS-PAGE - SALL2 overexpression 293T lysate (whole cell) (ab94229)
    ab94229 at 15μg/lane on an SDS-PAGE gel
  • Western blot - SALL2 overexpression 293T lysate (whole cell) (ab94229)
    Western blot - SALL2 overexpression 293T lysate (whole cell) (ab94229)
    All lanes : Anti-SALL2 antibody (ab55723) at 1/500 dilution

    Lane 1 : SALL2 overexpression 293T lysate (whole cell) (ab94229)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Protocols

  • Transfected Lysate Preparation Notes

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab94229? Please let us know so that we can cite the reference in this datasheet.

    ab94229 has not yet been referenced specifically in any publications.

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