Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (germ cell tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, pH6, 100C, 20min
Permeabilization
No
Specification
germ cell tumor
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: Dako diluent
Dilution
1/1600
Secondary antibody
Name
Non-Abcam antibody was used: Leica Refine anti-mouse anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
automated on Bond III various pretreatment regimens tested. Citrate gave best signal to background
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Submitted Jun 22 2015