Overview

  • Product name

    Anti-Sall4 antibody [SP289]
    See all Sall4 primary antibodies
  • Description

    Rabbit monoclonal [SP289] to Sall4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Sall4 aa 900-1000. The exact sequence is proprietary. (Near the C-terminus).
    Database link: Q9UJQ4

  • Positive control

    • IHC-P: Human seminoma, testis, and Human ovarian carcinoma tissue; FC: NCCIT cells; ICC: NCCIT cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab226756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary incubation for 10 minutes at room temperature.

Flow Cyt 1/20.
ICC/IF 1/10.

Target

  • Function

    Probable transcription factor.
  • Tissue specificity

    Expressed in testis.
  • Involvement in disease

    Defects in SALL4 are the cause of Duane-radial ray syndrome (DRRS) [MIM:607323]; also known as Okihiro syndrome. DRRS is a disorder characterized by the association of forearm malformations with Duane retraction syndrome.
    Defects in SALL4 are the cause of oculootoradial syndrome (OORS) [MIM:147750]. Oculootoradial syndrome is an autosomal dominant condition characterized by upper limbs anomalies (radial ray defects, carpal bones fusion), extraocular motor disturbances, congenital bilateral non-progressive mixed hearing loss. Other less consistent malformations include heart involvement, mild thrombocytopenia and leukocytosis (before age 50), shoulder girdle hypoplasia, imperforate anus, kidney malrotation or rectovaginal fistula. The IVIC syndrome is an allelic disorder of Duane-radial ray syndrome (DRRS) with a similar phenotype.
  • Sequence similarities

    Belongs to the sal C2H2-type zinc-finger protein family.
    Contains 7 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AA407717 antibody
    • AL022809 antibody
    • AW536104 antibody
    • C330011P20Rik antibody
    • C78083 antibody
    • C78563 antibody
    • dJ1112F19.1 antibody
    • DRRS antibody
    • HSAL4 antibody
    • Sal like 4 (Drosophila) antibody
    • Sal like 4 antibody
    • Sal like Protein 4 antibody
    • Sal-like protein 4 antibody
    • Sall4 antibody
    • SALL4_HUMAN antibody
    • Spalt like transcription factor 4 antibody
    • Tex20 antibody
    • Zinc finger protein 797 antibody
    • Zinc finger protein SALL4 antibody
    • ZNF797 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian carcinoma tissue sections labeling Sall4 with ab226756 at 1/100 dilution (1.23 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunocytochemistry/ Immunofluorescence analysis of NCCIT (human pluripotent embryonic carcinoma epithelial cell) cells labeling Sall4 with purified ab226756 at 1/10 (12.3 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow cytometry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) labeling Sall4 with purified ab226756 at 1/20 dilution (6.15 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.

  • Formalin-fixed, paraffin-embedded human seminoma tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human seminoma tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human testis tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human seminoma tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human testis tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

References

ab226756 has not yet been referenced specifically in any publications.

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