RecombinantRabMAb

Recombinant Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)
  • Immunocytochemistry/ Immunofluorescence - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)
  • Flow Cytometry - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)
  • Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP289] to Sall4 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Sall4 antibody [SP289] - BSA and Azide free
    See all Sall4 primary antibodies

  • Description

    Rabbit monoclonal [SP289] to Sall4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Sall4 aa 900-1000. The exact sequence is proprietary. (Near the C-terminus).
    Database link: Q9UJQ4

  • Positive control

    • IHC-P: Human seminoma, testis, and Human ovarian carcinoma tissue; Flow Cyt: NCCIT cells; ICC: NCCIT cells.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab245759 is the carrier-free version of ab226756. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab245759 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab245759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.Primary incubation for 10 minutes at room temperature.

 

Target

  • Function

    Probable transcription factor.

  • Tissue specificity

    Expressed in testis.

  • Involvement in disease

    Defects in SALL4 are the cause of Duane-radial ray syndrome (DRRS) [MIM:607323]; also known as Okihiro syndrome. DRRS is a disorder characterized by the association of forearm malformations with Duane retraction syndrome.
    Defects in SALL4 are the cause of oculootoradial syndrome (OORS) [MIM:147750]. Oculootoradial syndrome is an autosomal dominant condition characterized by upper limbs anomalies (radial ray defects, carpal bones fusion), extraocular motor disturbances, congenital bilateral non-progressive mixed hearing loss. Other less consistent malformations include heart involvement, mild thrombocytopenia and leukocytosis (before age 50), shoulder girdle hypoplasia, imperforate anus, kidney malrotation or rectovaginal fistula. The IVIC syndrome is an allelic disorder of Duane-radial ray syndrome (DRRS) with a similar phenotype.

  • Sequence similarities

    Belongs to the sal C2H2-type zinc-finger protein family.
    Contains 7 C2H2-type zinc fingers.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • AA407717 antibody
      • AL022809 antibody
      • AW536104 antibody
      • C330011P20Rik antibody
      • C78083 antibody
      • C78563 antibody
      • dJ1112F19.1 antibody
      • DRRS antibody
      • HSAL4 antibody
      • Sal like 4 (Drosophila) antibody
      • Sal like 4 antibody
      • Sal like Protein 4 antibody
      • Sal-like protein 4 antibody
      • Sall4 antibody
      • SALL4_HUMAN antibody
      • Spalt like transcription factor 4 antibody
      • Tex20 antibody
      • Zinc finger protein 797 antibody
      • Zinc finger protein SALL4 antibody
      • ZNF797 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian carcinoma tissue sections labeling Sall4 with ab226756 at 1/100 dilution (1.23 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab226756)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)

      Formalin-fixed, paraffin-embedded human testis tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab226756).

    • Immunocytochemistry/ Immunofluorescence - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)
      Immunocytochemistry/ Immunofluorescence - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)

      Immunocytochemistry/ Immunofluorescence analysis of NCCIT (human pluripotent embryonic carcinoma epithelial cell) cells labeling Sall4 with purified ab226756 at 1/10 (12.3 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab226756).

    • Flow Cytometry - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)
      Flow Cytometry - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)

      Flow cytometry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) labeling Sall4 with purified ab226756 at 1/20 dilution (6.15 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab226756).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)

      Formalin-fixed, paraffin-embedded human seminoma tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab226756).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)

      Formalin-fixed, paraffin-embedded human testis tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab226756).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)

      Formalin-fixed, paraffin-embedded human seminoma tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab226756).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)

      Formalin-fixed, paraffin-embedded human seminoma tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab226756).

    • Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)
      Anti-Sall4 antibody [SP289] - BSA and Azide free (ab245759)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab245759? Please let us know so that we can cite the reference in this datasheet.

    ab245759 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com