Overview

  • Product name

    Salmon Sperm ssDNA
  • General notes

    This product is a convenient, ready-to-use solution of salmon sperm DNA especially prepared for use in the preparation of pre-hybridization and hybridization solutions and as a DNA carrier in yeast transformation protocols and other related methods. This solution contains sheared single-stranded DNA molecules that can be used to block the non-specific attachment of probe DNA to the surface of a membrane (Southern) or to increase yeast transformation efficiency.

    Background: Prepared by a modification of the method of Emanuel and Chaikoff, JBC 203, 164 (1953) from salmon testes DNA by mechanical shearing and heat denaturation to an average size of 100 to 200 base pairs. To reverse any renaturation occurring during storage, this material should be briefly boiled and rapidly chilled prior to use. >75% native nucleic acid.

    Purity/Specifity: A260/A280: 1.85 % Hyperchromocity: 30.5.

  • Tested applications

    Suitable for: ChIP, In situ hybridization, Blockingmore details

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.60
    Constituents: 0.16% Tris HCl, 0.03% EDTA
  • Concentration information loading...
  • Research areas

  • Relevance

    The cell death process during apoptosis is essential for normal tissue homeostasis and cellular differentiation in multi cellular organisms. Single stranded DNA (ss DNA) is produced by nuclear fragmentation during the early stages of the apoptotic process.
  • Cellular localization

    Nuclear
  • Alternative names

    • Single stranded deoxyribose nucleic acid
    • Single stranded DNA

Applications

Our Abpromise guarantee covers the use of ab229278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
In situ hybridization Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration.

References

ab229278 has not yet been referenced specifically in any publications.

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