Overview

  • Product name
  • Description
    Rabbit polyclonal to Salmonella
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with Escherichia coli as determined by immunofluorescence using acetone fixed suspensions of the bacteria. Reacts with S. enteriditis, S. typhimurium and S. heidelburg. Not yet tested in other species.
  • Immunogen

    Tissue/ cell preparation: Mixture of S. enteriditis, S. typhimurium and S. heidelburg.

  • General notes


    This antibody is unabsorbed for related Enterobacteriaceae.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purification notes
    The purification method used is proprietary. This antibody is greater than 95% pure.
  • Primary antibody notes
    This antibody is unabsorbed for related Enterobacteriaceae.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab35156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/20 - 1/1000. PubMed: 28123538
ELISA 1/100 - 1/2000.

Target

  • Relevance
    The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

References

This product has been referenced in:
  • Li CX  et al. 'Obligate' anaerobic Salmonella strain YB1 suppresses liver tumor growth and metastasis in nude mice. Oncol Lett 13:177-183 (2017). ICC/IF . Read more (PubMed: 28123538) »
  • Brooks AB  et al. MYO6 is targeted bySalmonellavirulence effectors to trigger PI3-kinase signaling and pathogen invasion into host cells. Proc Natl Acad Sci U S A 114:3915-3920 (2017). Read more (PubMed: 28348208) »
See all 10 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer

I am very pleased to hear you would like to accept our offer and test ab35156 in sELISA. This code will give you: 1 free primary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forsELISA and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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Question
Answer

Thank you for your query.

Unfortunately we currently do not have any data (either empirical or from literature) to support reactivity to S. infantis. However, according to the following document (http://nih.dmsc.moph.go.th/aboutus/media/antigenic%20formula%20of%20Salmonella.pdf), S. Infantis shares H antigens with S. Heidelberg and S. Typhimurium (two of the serovars used in the immunogen preparation). Based on this, there is a good chance the antibody may work with S. Infantis. However, please bear in mind that this species has not been tested and we will not be able to guarantee good results.

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Question
Answer

Thank you for your enquiry. The originator of the antibody has informed me that the actual concentration of this product is between 4 and 5 mg/ml. This is the most specific information I was able to obtain. I hope this information helps. Please do not hesitate to contact us again if you need anything further.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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