Overview

  • Product name

    Anti-Salmonella antibody (Biotin)
    See all Salmonella primary antibodies
  • Description

    Rabbit polyclonal to Salmonella (Biotin)
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with Salmonella sp.
  • Immunogen

    Mixture of Salmonella enteriditis, Salmonella typhimurium and Salmonella heidelburg (O and H antigens).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab69255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • Relevance

    The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

References

This product has been referenced in:

  • Wang KY  et al. Disposable syringe-based visual immunotest for pathogenic bacteria based on the catalase mimicking activity of platinum nanoparticle-concanavalin A hybrid nanoflowers. Mikrochim Acta 186:57 (2019). Read more (PubMed: 30617909) »
  • Cai G  et al. A Fluidic Device for Immunomagnetic Separation of Foodborne Bacteria Using Self-Assembled Magnetic Nanoparticle Chains. Micromachines (Basel) 9:N/A (2018). Read more (PubMed: 30486364) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Bacteria Cell (Salmonella infected liver)
Specification
Salmonella infected liver
Permeabilization
Yes - 0.25% Triton X-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 09 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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