Reacts with Salmonella species.
Not yet tested in other species.
Tissue/ cell preparation (Mixture of S. enteriditis, S. typhimurium, and S. heidelburg).
Purified IgG fraction of the antiserum covalent coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF: This antibody has been tested in IFA using organisms fixed on slides. After the organism suspension is spotted on glass slides and air dried, the slides are fixed by immersion in 100% acetone for 5-10 minutes at room temperature. This antibody conjugate has worked well at dilutions of 1:40 - 1:80.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.