Overview

  • Product name
    Anti-Salmonella group antigen antibody (HRP)
  • Description
    Rabbit polyclonal to Salmonella group antigen (HRP)
  • Host species
    Rabbit
  • Conjugation
    HRP
  • Specificity
    ab53299 is polyvalent for Salmonella "O" and "H" antigens.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with Salmonella species.
  • Immunogen

    Mixture of S. enteriditis, S. typhimurium and S. heidelburg.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.002% Thimerosal (merthiolate)
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purification notes
    This antibody is greater than 95% pure.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-Fr
  • Application notes
    ELISA: 1/200 - 1/1000.
    IHC-Fr: 1/20 - 1/200.
    WB: 1/20 - 1/200.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

    References

    This product has been referenced in:
    • Hulme HE  et al. Mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during Salmonella Typhimurium infection. Sci Rep 7:2786 (2017). Read more (PubMed: 28584281) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Question
    Answer

    I am very pleased to hear you would like to accept our offer and test ab35156 in sELISA. This code will give you: 1 free primary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forsELISA and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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