Overview

  • Product name
    Anti-Salmonella typhimurium LPS antibody [1E6]
  • Description
    Mouse monoclonal [1E6] to Salmonella typhimurium LPS
  • Host species
    Mouse
  • Specificity
    This antibody is directed against lipopolisaccharides of Salmonella typhimurium
  • Tested applications
    Suitable for: Flow Cyt, WB, Dot blot, ELISA, ICC/IF, IHC-Frmore details
  • Immunogen

    Lipopolysaccharides of Salmonella typhimurium

  • General notes

    This antibody is used for the detection of LPS of Salmonella typhimurium.

    This product was changed from ascites to tissue culture supernatant on 16th July 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab8274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 17442963

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    Salmonellae are Gram-negative , facultatively anaerobic bacteria of the family Enterobacteriaceae, made up of nonspore-forming rods, usually motile with flagella. Salmonella enterica serovar Typhimurium is the among the most common Salmonella serovars causing Salmonellosis food poisoning. S. typhimurium has a less alarming public image than S.typhi (which causes typhoid), but is a bigger health problem worldwide. Salmonellae possess 3 major antigens; the "H" or flagellar antigen (phase 1 & 2), the "O" or somatic antigen (part of the LPS moiety) and the "Vi" or capsular antigen (referred to as "K" in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram-negative bacteria. This LPS is composed of an "O" polysaccharide ("O" antigen) an "R" core and the endotoxic inner "Lipid A". Endotoxins evoke fever and can activate complement, kinin and clotting factors.
  • Alternative names
    • S. typhimurium antibody
    • Salmonella typhimurium antibody
    • SALMONELLA TYPHIMURIUM LPS FACTOR 4 antibody

Images

  • Electron microscopy image of ultrathin cryosections of Salmonella paratyphi using ab8274 (1/100). For further experimental details please refer to abreview.

    See Abreview

  • All lanes : Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274) at 1/1000 dilution

    Lane 1 : Whole cell polysaccharides from S. Enteritidis 27655-3b
    Lane 2 : Whole cell polysaccharides from S. Enteritidis Sal8
    Lane 3 : Whole cell polysaccharides from S. Typhimurium ATCC 14028s
    Lane 4 : Whole cell polysaccharides from S. Typhimurium SL1344

    Lysates/proteins at 100000000 cells per lane.

    Secondary
    All lanes : AP conjugated goat anti-mouse polyclonal at 1/4000 dilution


    Gels were (A) silver-stained or (B) Western blotted using the Anti-Salmonella typhimurium 0-4 antibody [1E6] (ab8274) as the primary antibody. Although equal amounts of cellular material were detected by silver staining, only the S. Typhimurium samples were recognized by the 1E6 antibody on the corresponding Western blot.

    See Abreview

References

This product has been referenced in:
  • Kim K  et al. Cell mass-dependent expression of an anticancer protein drug by tumor-targetedSalmonella. Oncotarget 9:8548-8559 (2018). Read more (PubMed: 29492216) »
  • Lim D  et al. The hepcidin-ferroportin axis controls the iron content of Salmonella-containing vacuoles in macrophages. Nat Commun 9:2091 (2018). Read more (PubMed: 29844422) »
See all 9 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer


Unfortunately, we do not carry the biotinylated forms of these 2 antibodies, but we do carry biotinylation kits (e.g. https://www.abcam.com/EasyLink-Biotin-type-A-Conjugation-Kit-3-x-100µg-Biotin-ab102864.html - other sizes exist as well) if you like to biotinylate the antibodies on your own.

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Answer

We have 5 antibodies to Salmonella typhimurium:

ab69238 - was raised against the whole bacteria thus we do not know where the antibody would bind.

ab8274 and ab65922: both were raised against LPS of the bacterium which I suppose is located in the bacterial wall and not the flagellum - this should work (https://www.abcam.com/salmonella-typhimurium-0-4-antibody-1e6-ab8274.html)
(https://www.abcam.com/salmonella-typhimurium-lps-antibody-1e6-ab65922.html)

ab63901: was raised against the bacteria, but known to be reactive with the LPS - thus, it should work.

ab128709: raised against purified LPS from Salmonella typhimurium and reacts with an unidentified LPS determinant of Salmonella typhimurium - thus it should work for your purposes (https://www.abcam.com/index.html?datasheet=128709).

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Salmonella Typhimurium Cell lysate - other (Whole cell polysaccharides)
Loading amount
1e+008 cells
Specification
Whole cell polysaccharides
Treatment
Proteinase K (0.5mg/mL) for 1 h at 65C
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C

Aaron White

Verified customer

Submitted Jan 27 2012

Answer

Thank you for contacting Abcam.

I have no added both Salmonella Typhimurium and Salmonella Enteritidis to our list of species and so you should now be able to submit anAbreview for this antibody.

If there is anything else I can help you with, please let me know.

Read More

Question
Answer

DISCOUNT CODE: ****** Expiration date:****** I am very pleased to hear you would like to accept our offer and test ab8274 in western blot. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for western blot and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Salmonella paratyphi Tissue sections (Salmonella ultrathin cryosections)
Specification
Salmonella ultrathin cryosections
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted May 29 2008

Answer

Thank you for your enquiry regarding our Salmonella typhimurium, Listeria monocytogenes, or Campylobacter jejuni antibodies. For Listeria monocytogenes detection we recommend pair LZH1 (ab11439) – LZF7 (ab11440)(capture – conjugate) for sandwich immunoassay. (The best pair for sandwich ELISA was selected among the antibodies by testing all possible pairs). Unfortunately we have available only one antibody clone for the detection of Salmonella typhimurium. However, we are also offering antibodies for the detection of A, B and D-group specific salmonella O-antigens. We have information from a customer that they have used successfully the pair 10D9 (ab8272) – 5D12A (ab8273) (capture – conjugate) in immunochromatography for the detection of salmonella from food. The detection antibodies are available in conjugated form (e.g. hrp-conjugated). We are very sorry we do not have references for those antibodies. Here is the recommended protocol for Sandwich ELISA: Coating: Capture MAbs, 2.5 µg/ml in 0.1 M carbonate buffer, pH 9.3, overnight at + 4 C. Antigen: L. monocytogenes OM fraction or cell suspension, 1 h, 37 C. Conjugate: Conjugated MAbs in PBS-AT, 1 h, 37 C. Substrate: TMB, 15 min Using pair LZH1 – LZF7 the detection limits 0.3 ng/ml for L. monocytogenes OM fraction and 100 cells per well for L. monocytogenes cell suspension have been achieved. For Listeria monocytogenes detection we recommend pair LZH1 (ab11439) – LZF7 (capture – conjugate) for sandwich immunoassay. (The best pair for sandwich ELISA was selected among the antibodies by testing all possible pairs). Unfortunately we have available only one antibody clone for the detection of Salmonella typhimurium. However, we are also offering antibodies for the detection of A, B and D-group specific salmonella O-antigens. We have information from a customer that they have used successfully the pair 10D9 (ab8272) – 5D12A (ab8273) (capture – conjugate) in immunochromatography for the detection of salmonella from food. The detection antibodies are available in conjugated form (e.g. hrp-conjugated). We are very sorry we do not have references for those antibodies. Sandwich ELISA Protocol: Coating: Capture MAbs, 2.5 µg/ml in 0.1 M carbonate buffer, pH 9.3, overnight at + 4 C. Antigen: L. monocytogenes OM fraction or cell suspension, 1 h, 37 C. Conjugate: Conjugated MAbs in PBS-AT, 1 h, 37 C. Substrate: TMB, 15 min Using pair LZH1 – LZF7 the detection limits 0.3 ng/ml for L. monocytogenes OM fraction and 100 cells per well for L. monocytogenes cell suspension have been achieved. Please do not hesitate to contact us again if you need further assistance or the HRP conjugated detection antibodies and we will add them to the catalogue and provide you with their catalogue number.

Read More

Answer

Both monoclonal antibodies recognize heat-killed Salmonella typhimurium. We have used the monoclonal antibodies (Mab) in ELISA according to the following scheme: antigen coating - Mab - Anti-mouse-IgG (conjugated), not in sandwich ELISA. The antibodies may be used for salmonella typhimurium detection in dot-blot, ELISA and immunofluorescence. Other applications have not been tested. Both clones do not recognise: S.paratyphi A, S.choleraesuis, S.newport, S. enteiditis, S. anatum, S. elandia, E.coli 055:B5, E.coli K12, Klebsiela pneum, Proteus vulgaris. Ab8274 has been shown to have a very broad cross-reactivity range recognising E.coli 1243 and Listeria monocytogenes species, being a potential positive control antibody for a variety of assays.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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