Key features and details
- Mouse monoclonal [1E6] to Salmonella typhimurium LPS
- Suitable for: WB, Dot blot, ELISA, ICC/IF, IHC-Fr
- Reacts with: Salmonella typhimurium
- Isotype: IgG1
Product nameAnti-Salmonella typhimurium LPS antibody [1E6]
DescriptionMouse monoclonal [1E6] to Salmonella typhimurium LPS
SpecificityThis antibody is directed against lipopolisaccharides of Salmonella typhimurium
Tested applicationsSuitable for: WB, Dot blot, ELISA, ICC/IF, IHC-Frmore details
Species reactivityReacts with: Salmonella typhimurium
Lipopolysaccharides of Salmonella typhimurium
This antibody is used for the detection of LPS of Salmonella typhimurium.
This product was changed from ascites to tissue culture supernatant on 16th July 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein A purified
Primary antibody notesThis antibody is used for the detection of LPS of Salmonella typhimurium
Light chain typeunknown
Our Abpromise guarantee covers the use of ab8274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
RelevanceSalmonellae are Gram-negative , facultatively anaerobic bacteria of the family Enterobacteriaceae, made up of nonspore-forming rods, usually motile with flagella. Salmonella enterica serovar Typhimurium is the among the most common Salmonella serovars causing Salmonellosis food poisoning. S. typhimurium has a less alarming public image than S.typhi (which causes typhoid), but is a bigger health problem worldwide. Salmonellae possess 3 major antigens; the "H" or flagellar antigen (phase 1 & 2), the "O" or somatic antigen (part of the LPS moiety) and the "Vi" or capsular antigen (referred to as "K" in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram-negative bacteria. This LPS is composed of an "O" polysaccharide ("O" antigen) an "R" core and the endotoxic inner "Lipid A". Endotoxins evoke fever and can activate complement, kinin and clotting factors.
- S. typhimurium antibody
- Salmonella typhimurium antibody
- SALMONELLA TYPHIMURIUM LPS FACTOR 4 antibody
Electron microscopy image of ultrathin cryosections of Salmonella paratyphi using ab8274 (1/100). For further experimental details please refer to abreview.
All lanes : Anti-Salmonella typhimurium LPS antibody [1E6] (ab8274) at 1/1000 dilution
Lane 1 : Whole cell polysaccharides from S. Enteritidis 27655-3b
Lane 2 : Whole cell polysaccharides from S. Enteritidis Sal8
Lane 3 : Whole cell polysaccharides from S. Typhimurium ATCC 14028s
Lane 4 : Whole cell polysaccharides from S. Typhimurium SL1344
Lysates/proteins at 100000000 cells per lane.
All lanes : AP conjugated goat anti-mouse polyclonal at 1/4000 dilution
Gels were (A) silver-stained or (B) Western blotted using the Anti-Salmonella typhimurium 0-4 antibody [1E6] (ab8274) as the primary antibody. Although equal amounts of cellular material were detected by silver staining, only the S. Typhimurium samples were recognized by the 1E6 antibody on the corresponding Western blot.
ab8274 has been referenced in 16 publications.
- Tewari Kumar P et al. Digital Microfluidics for Single Bacteria Capture and Selective Retrieval Using Optical Tweezers. Micromachines (Basel) 11:N/A (2020). PubMed: 32183431
- Xu Y et al. A Bacterial Effector Reveals the V-ATPase-ATG16L1 Axis that Initiates Xenophagy. Cell 178:552-566.e20 (2019). PubMed: 31327526
- Kolodziejek AM et al. Salmonella Translocated Effectors Recruit OSBP1 to the Phagosome to Promote Vacuolar Membrane Integrity. Cell Rep 27:2147-2156.e5 (2019). PubMed: 31091452
- Kim K et al. Cell mass-dependent expression of an anticancer protein drug by tumor-targetedSalmonella. Oncotarget 9:8548-8559 (2018). PubMed: 29492216
- Lim D et al. The hepcidin-ferroportin axis controls the iron content of Salmonella-containing vacuoles in macrophages. Nat Commun 9:2091 (2018). PubMed: 29844422