Anti-SAMD12 antibody (ab121831)
Key features and details
- Rabbit polyclonal to SAMD12
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SAMD12 antibody -
Description
Rabbit polyclonal to SAMD12 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: LHCGLNPRGI DHPAHAEGIK LQIEGEGVES QSIKNKNFQK VPDQKGTPKR LQAEAETAKS ATVKLSKPVA LWTQQDVCKW LKKHCPNQYQ IYSESF, corresponding to amino acids 6-101 of Human SAMD12 (UniProt ID:Q8N8I0).
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Positive control
- Human brain tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Sequence similarities
Contains 1 SAM (sterile alpha motif) domain. - Information by UniProt
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Database links
- Entrez Gene: 401474 Human
- SwissProt: Q8N8I0 Human
- Unigene: 359393 Human
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Alternative names
- SAM domain-containing protein 12 antibody
- SAM12_HUMAN antibody
- Samd12 antibody
- Sterile alpha motif domain-containing protein 12 antibody
Images
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Immunohistochemical analysis of human cerebral cortex tissue labelling SAMD12 using ab121831 at a 1/200 dilution. Cytoplasmic staining observed in human neuronal cells.
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Immunohistochemical analysis of human pancreas tissue labelling SAMD12 using ab121831 at a 1/200 dilution. Cytoplasmic staining observed in human islets of Langerhans.
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Immunohistochemical analysis of human fallopian tube tissue labelling SAMD12 using ab121831 at a 1/200 dilution. Cytoplasmic staining observed in human glandular cells.
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Immunohistochemical analysis of human colon tissue labelling SAMD12 using ab121831 at a 1/200 dilution. Cytoplasmic staining observed in human glandular cells.
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Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm and golgi apparatus. Recommended concentration of ab121831 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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All lanes : Anti-SAMD12 antibody (ab121831) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over expression Lysate (co-expressed with a C terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121831 has not yet been referenced specifically in any publications.