Key features and details
- Rabbit polyclonal to SAMD12
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SAMD12 antibody
DescriptionRabbit polyclonal to SAMD12
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- Human stomach tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
Sequence similaritiesContains 1 SAM (sterile alpha motif) domain.
- Information by UniProt
- SAM domain-containing protein 12 antibody
- SAM12_HUMAN antibody
- Samd12 antibody
- Sterile alpha motif domain-containing protein 12 antibody
Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm and golgi apparatus. Recommended concentration of ab121831 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-SAMD12 antibody (ab121831) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over expression Lysate (co-expressed with a C terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
ab121831, at a 1/100 dilution, staining SAMD12 in paraffin embedded Human stomach tissue by Immunohistochemistry.
ab121831 has not yet been referenced specifically in any publications.