Anti-SAMD3 antibody (ab122028)

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Overview

  • Product name

    Anti-SAMD3 antibody

  • Description

    Rabbit polyclonal to SAMD3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment corresponding to amino acids 221-303 of Human SAMD3 isoform 1 (Q8N6K7)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human pancreas tissue. Total protein lysate from RT-4 and U-251 MG cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
WB 1/250 - 1/500.

Target

Images

  • Western blot - Anti-SAMD3 antibody (ab122028)
    Western blot - Anti-SAMD3 antibody (ab122028)
    Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD3 antibody (ab122028)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMD3 antibody (ab122028)
    ab122028 at 1/40 dilution staining SAMD3 in paraffin embedded Human pancreas tissue. This image shows strong cytoplasmic positivity in exocrine glandular cells.
  • Western blot - Anti-SAMD3 antibody (ab122028)
    Western blot - Anti-SAMD3 antibody (ab122028)
    All lanes : Anti-SAMD3 antibody (ab122028) at 1/250 dilution

    Lane 1 : Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11

    Lane 2 : RT-4
    Lane 3 : U-251 MG

    Lane 4 : Human Plasma

    Lane 5 : Human Liver
    Lane 6 : Human Tonsil

References

This product has been referenced in:

  • Cheng Y  et al. MicroRNA-30e regulates TGF-ß-mediated NADPH oxidase 4-dependent oxidative stress by Snai1 in atherosclerosis. Int J Mol Med 43:1806-1816 (2019). Read more (PubMed: 30816428) »
  • Liu M  et al. Overexpression of miR-422a inhibits cell proliferation and invasion, and enhances chemosensitivity in osteosarcoma cells. Oncol Rep 36:3371-3378 (2016). Read more (PubMed: 27779704) »
See all 2 Publications for this product

Publishing research using ab122028? Please let us know so that we can cite the reference in this datasheet.

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