Key features and details
- Rabbit polyclonal to SAMD9
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SAMD9 antibody
See all SAMD9 primary antibodies
DescriptionRabbit polyclonal to SAMD9
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- Human thyroid gland tissue; RT4 and U 251 MG cell lysates; A431 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250 - 1/500.|
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
Tissue specificityWidely expressed. Very low levels in skeletal muscle. Not detected in fetal brain. Down-regulated in aggressive fibromatosis, as well as in breast and colon cancers.
Involvement in diseaseDefects in SAMD9 are the cause of normophosphatemic familial tumoral calcinosis (NFTC) [MIM:610455]. NFTC is an uncommon life-threatening disorder characterized by massive periarticular, and seldom visceral, deposition of calcified tumors.
Sequence similaritiesContains 1 SAM (sterile alpha motif) domain.
- Information by UniProt
- C7orf5 antibody
- DRIF1 antibody
- expressed in aggressive fibromatosis antibody
All lanes : Anti-SAMD9 antibody (ab121663) at 1/250 dilution
Lane 1 : RT4 cell lysates
Lane 2 : U251MG cell lysates
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysates
Lane 5 : Human tonsil lysates
Developed using the ECL technique.
ab121663, at 1/30 dilution, staining SAMD9 in paraffin-embedded Human thyroid gland tissue by Immunohistochemistry.
ab121663 at 4 µg/ml staining SAMD9 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
ab121663 has not yet been referenced specifically in any publications.