Overview

  • Product name

    Anti-SAMD9 antibody [EPR13603]
    See all SAMD9 primary antibodies
  • Description

    Rabbit monoclonal [EPR13603] to SAMD9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SAMD9 aa 50-200. The exact sequence is proprietary.
    Database link: Q5K651

  • Positive control

    • WB: A431 and MCF7 cell lysates. FC: A431 and MCF7 cells. ICC/FC: A431 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13603
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/30 - 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/10000. Predicted molecular weight: 184 kDa.
ICC/IF 1/50 - 1/100.

Target

  • Tissue specificity

    Widely expressed. Very low levels in skeletal muscle. Not detected in fetal brain. Down-regulated in aggressive fibromatosis, as well as in breast and colon cancers.
  • Involvement in disease

    Defects in SAMD9 are the cause of normophosphatemic familial tumoral calcinosis (NFTC) [MIM:610455]. NFTC is an uncommon life-threatening disorder characterized by massive periarticular, and seldom visceral, deposition of calcified tumors.
  • Sequence similarities

    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • C7orf5 antibody
    • DRIF1 antibody
    • expressed in aggressive fibromatosis antibody
    • KIAA2004 antibody
    • NFTC antibody
    • OEF1 antibody
    • OEF2 antibody
    • SAM domain-containing protein 9 antibody
    • SAMD9 antibody
    • SAMD9_HUMAN antibody
    • sterile alpha motif domain containing 9 antibody
    • Sterile alpha motif domain-containing protein 9 antibody
    see all

Images

  • Anti-SAMD9 antibody [EPR13603] (ab180575) at 1/1000 dilution (Purified) + MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 184 kDa
    Observed band size: 184 kDa

  • Flow Cytometry analysis of MCF-7 (Human breast adenocarcinoma epithelial cell) cells labeling SAMD9 with purified ab180575 at 1/30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling SAMD9 with purified ab180575 at 1:50 dilution (5.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with none. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • All lanes : Anti-SAMD9 antibody [EPR13603] (ab180575) at 1/10000 dilution (unpurified)

    Lane 1 : A431 cell lysate with NFDM/TBST
    Lane 2 : MCF-7 cell lysate with NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Goat Anti-rabbit HRP at 1/1000 dilution

    Predicted band size: 184 kDa

  • Immunofluorescence analysis of 4% paraformaldehyde-A431 cells labeling SAMD9 with ab180575 (unpurified) (green) at 1/100 dilution. Dapi counterstain (blue). Goat anti rabbit IgG (Dylight 488) at a dilution of 1/200 was used as a secondary antibody.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed A431 cells labeling SAMD9 with ab180575 (unpurified) at 1/50 dilution, or Rabbit monoclonal IgG Isotype control.

References

This product has been referenced in:

See all 2 Publications for this product

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