Overview

  • Product name

  • Description

    Mouse polyclonal to SAMHD1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length SAMHD1 protein (Human)

  • Positive control

    • SAMHD1 transfected 293T cell lysate and human liver tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 23076149

Target

  • Function

    Putative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling.
  • Tissue specificity

    Expressed in heart, skeletal muscle, spleen, liver, small intestine, placenta, lung and peripheral blood leukocytes. No expression is seen in brain and thymus.
  • Involvement in disease

    Defects in SAMHD1 are the cause of Aicardi-Goutieres syndrome type 5 (AGS5) [MIM:612952]. A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood.
  • Sequence similarities

    Belongs to the SAMHD1 family.
    Contains 1 HD domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CHBL2 antibody
    • DCIP antibody
    • Dendritic cell derived IFNG induced protein antibody
    • Dendritic cell-derived IFNG-induced protein antibody
    • Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 antibody
    • dNTPase antibody
    • HD domain containing 1 antibody
    • HDDC1 antibody
    • Mg11 antibody
    • Monocyte protein 5 antibody
    • MOP 5 antibody
    • MOP-5 antibody
    • MOP5 antibody
    • OTTHUMP00000030889 antibody
    • SAM domain and HD domain 1 antibody
    • SAM domain and HD domain containing protein 1 antibody
    • SAM domain and HD domain-containing protein 1 antibody
    • SAMH1_HUMAN antibody
    • Samhd1 antibody
    • SBBI88 antibody
    see all

Images

  • Anti-SAMHD1 antibody (ab67820) at 1/500 dilution + human liver tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa

  • All lanes : Anti-SAMHD1 antibody (ab67820) at 1/500 dilution

    Lane 1 : SAMHD1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa

  • Immunofluorescence analysis of HeLa cells expressing SAMHD1 fused to murine NIH3T3 (3T3) cells, staining SAMHD1 with ab67820. HeLa cells expressing the SAMHD1 were fused to murine NIH3T3 cells with PEG to induce heterokaryon formation. Cells were fixed with 4% formalin, permeabilized with 0.5% Triton X-100 before incubating with primary antibody

References

This product has been referenced in:

  • Wing PA  et al. A dual role for SAMHD1 in regulating HBV cccDNA and RT-dependent particle genesis. Life Sci Alliance 2:N/A (2019). Read more (PubMed: 30918010) »
  • Antonucci JM  et al. SAMHD1 Impairs HIV-1 Gene Expression and Negatively Modulates Reactivation of Viral Latency in CD4+ T Cells. J Virol 92:N/A (2018). Read more (PubMed: 29793958) »
See all 26 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Testis)
Antigen retrieval step
None
Permeabilization
Yes - Triton X-100
Specification
Testis
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde

一恵 垣内

Verified customer

Submitted Jun 09 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1)
Loading amount
25 µg
Specification
THP-1
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Apr 17 2012

Answer

Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this product. All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience. This is a very first complaint about this antibody so we are not aware of any quality-related issues. I appreciate very much that you have attached the WB results to this e-mail and provided some further details of the experiments. It is very clear to me that the two batches (GR19443-2 and GR52366-1) performed differently. At this stage, I am unable to identify the source of this problem but the two batches represent two different purifications. As our Abpromise applies to this complaint, this is to confirm that I have placed a new order for you for one vial of ab67820 (different batch: 1155439) as a free of charge replacement. I hope the second vial will work as it is expected, and please do let me know how you are getting on with this product.

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