Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-SAMHD1 antibody [EPR13034] - N-terminal (ab191420)

Overview

  • Product name

    Anti-SAMHD1 antibody [EPR13034] - N-terminal
    See all SAMHD1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13034] to SAMHD1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SAMHD1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q9Y3Z3

  • Positive control

    • THP1, Daudi, HeLa and HepG2 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab191420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).

Target

  • Function

    Putative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling.
  • Tissue specificity

    Expressed in heart, skeletal muscle, spleen, liver, small intestine, placenta, lung and peripheral blood leukocytes. No expression is seen in brain and thymus.
  • Involvement in disease

    Defects in SAMHD1 are the cause of Aicardi-Goutieres syndrome type 5 (AGS5) [MIM:612952]. A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood.
  • Sequence similarities

    Belongs to the SAMHD1 family.
    Contains 1 HD domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CHBL2 antibody
    • DCIP antibody
    • Dendritic cell derived IFNG induced protein antibody
    • Dendritic cell-derived IFNG-induced protein antibody
    • Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 antibody
    • dNTPase antibody
    • HD domain containing 1 antibody
    • HDDC1 antibody
    • Mg11 antibody
    • Monocyte protein 5 antibody
    • MOP 5 antibody
    • MOP-5 antibody
    • MOP5 antibody
    • OTTHUMP00000030889 antibody
    • SAM domain and HD domain 1 antibody
    • SAM domain and HD domain containing protein 1 antibody
    • SAM domain and HD domain-containing protein 1 antibody
    • SAMH1_HUMAN antibody
    • Samhd1 antibody
    • SBBI88 antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: SAMHD1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HepG2 cell lysate (20 µg)
    Lane 4: HeLa cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab191420 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab191420 was shown to recognize SAMHD1 when SAMHD1 knockout samples were used, along with additional cross-reactive bands. Wild-type and SAMHD1 knockout samples were subjected to SDS-PAGE. ab191420 and ab8245 (loading control to GAPDH) dilted to 1/500 and 1/10000 respectively were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Anti-SAMHD1 antibody [EPR13034] - N-terminal (ab191420) at 1/10000 dilution + THP1 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 72 kDa

  • All lanes : Anti-SAMHD1 antibody [EPR13034] - N-terminal (ab191420) at 1/1000 dilution

    Lane 1 : Daudi cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 72 kDa

References

ab191420 has not yet been referenced specifically in any publications.

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