Product nameAnti-SAMHD1 antibody [EPR13040(B)] - C-terminal
See all SAMHD1 primary antibodies
DescriptionRabbit monoclonal [EPR13040(B)] to SAMHD1 - C-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide within Human SAMHD1 aa 600 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: Q9Y3Z3
- THP1, K562, HeLa and MCF7 cell lysates. Human pancreas and tonsil tissue. MCF7 and THP1 cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab177462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 72 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPutative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling.
Tissue specificityExpressed in heart, skeletal muscle, spleen, liver, small intestine, placenta, lung and peripheral blood leukocytes. No expression is seen in brain and thymus.
Involvement in diseaseDefects in SAMHD1 are the cause of Aicardi-Goutieres syndrome type 5 (AGS5) [MIM:612952]. A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood.
Sequence similaritiesBelongs to the SAMHD1 family.
Contains 1 HD domain.
Contains 1 SAM (sterile alpha motif) domain.
- Information by UniProt
- CHBL2 antibody
- DCIP antibody
- Dendritic cell derived IFNG induced protein antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SAMHD knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab177462 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab177462 was shown to recognize SAMHD1 when SAMHD1 knockout samples were used, along with additional cross-reactive bands. Wild-type and SAMHD1 knockout samples were subjected to SDS-PAGE. ab177462 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling SAMHD1 with ab177462 at 1/100 dilution.
Immunofluorescence analysis of MCF7 cells labeling SAMHD1 with ab177462 at 1/100 dilution.
Flow cytometric analysis of permeabilized THP1 cells labeling SAMHD1 (red) with ab177462 at 1/10 dilution or rabbit IgG (negative) (green).
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling SAMHD1 with ab177462 at 1/100 dilution.
All lanes : Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal (ab177462) at 1/10000 dilution
Lane 1 : THP1 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 72 kDa
ab177462 has not yet been referenced specifically in any publications.