Validated using a knockout cell line
Recombinant
RabMAb

Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal (ab177462)

Overview

  • Product name
    Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal
    See all SAMHD1 primary antibodies
  • Description
    Rabbit monoclonal [EPR13040(B)] to SAMHD1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SAMHD1 aa 600 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9Y3Z3

  • Positive control
    • THP1, K562, HeLa and MCF7 cell lysates. Human pancreas and tonsil tissue. MCF7 and THP1 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 72 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Putative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling.
    • Tissue specificity
      Expressed in heart, skeletal muscle, spleen, liver, small intestine, placenta, lung and peripheral blood leukocytes. No expression is seen in brain and thymus.
    • Involvement in disease
      Defects in SAMHD1 are the cause of Aicardi-Goutieres syndrome type 5 (AGS5) [MIM:612952]. A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood.
    • Sequence similarities
      Belongs to the SAMHD1 family.
      Contains 1 HD domain.
      Contains 1 SAM (sterile alpha motif) domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CHBL2 antibody
      • DCIP antibody
      • Dendritic cell derived IFNG induced protein antibody
      • Dendritic cell-derived IFNG-induced protein antibody
      • Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 antibody
      • dNTPase antibody
      • HD domain containing 1 antibody
      • HDDC1 antibody
      • Mg11 antibody
      • Monocyte protein 5 antibody
      • MOP 5 antibody
      • MOP-5 antibody
      • MOP5 antibody
      • OTTHUMP00000030889 antibody
      • SAM domain and HD domain 1 antibody
      • SAM domain and HD domain containing protein 1 antibody
      • SAM domain and HD domain-containing protein 1 antibody
      • SAMH1_HUMAN antibody
      • Samhd1 antibody
      • SBBI88 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: SAMHD knockout HAP1 cell lysate (20 µg)
      Lane 3: K562 cell lysate (20 µg)
      Lanes 1 - 3: Merged signal (red and green). Green - ab177462 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab177462 was shown to recognize SAMHD1 when SAMHD1 knockout samples were used, along with additional cross-reactive bands. Wild-type and SAMHD1 knockout samples were subjected to SDS-PAGE. ab177462 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling SAMHD1 with ab177462 at 1/100 dilution.

    • Immunofluorescence analysis of MCF7 cells labeling SAMHD1 with ab177462 at 1/100 dilution.

    • Flow cytometric analysis of permeabilized THP1 cells labeling SAMHD1 (red) with ab177462 at 1/10 dilution or  rabbit IgG (negative) (green).

    • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling SAMHD1 with ab177462 at 1/100 dilution.

    • All lanes : Anti-SAMHD1 antibody [EPR13040(B)] - C-terminal (ab177462) at 1/10000 dilution

      Lane 1 : THP1 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 72 kDa

    References

    ab177462 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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