Overview

  • Product name
    Anti-SAMHD1 antibody [OTI1A1]
    See all SAMHD1 primary antibodies
  • Description
    Mouse monoclonal [OTI1A1] to SAMHD1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, Monkey
  • Immunogen

    Recombinant full length protein corresponding to Human SAMHD1 aa 1-626. Produced in HEK-293T cells (NP_056289).
    Sequence:

    MQRADSEQPSKRPRCDDSPRTPSNTPSAEADWSPGLELHPDYKTWGPEQV CSFLRRGGFEEPVLLKNIRENEITGALLPCLDESRFENLGVSSLGERKKL LSYIQRLVQIHVDTMKVINDPIHGHIELHPLLVRIIDTPQFQRLRYIKQL GGGYYVFPGASHNRFEHSLGVGYLAGCLVHALGEKQPELQISERDVLCVQ IAGLCHDLGHGPFSHMFDGRFIPLARPEVKWTHEQGSVMMFEHLINSNGI KPVMEQYGLIPEEDICFIKEQIVGPLESPVEDSLWPYKGRPENKSFLYEI VSNKRNGIDVDKWDYFARDCHHLGIQNNFDYKRFIKFARVCEVDNELRIC ARDKEVGNLYDMFHTRNSLHRRAYQHKVGNIIDTMITDAFLKADDYIEIT GAGGKKYRISTAIDDMEAYTKLTDNIFLEILYSTDPKLKDAREILKQIEY RNLFKYVGETQPTGQIKIKREDYESLPKEVASAKPKVLLDVKLKAEDFIV DVINMDYGMQEKNPIDHVSFYCKTAPNRAIRITKNQVSQLLPEKFAEQLI RVYCKKVDRKSLYAARQYFVQWCADRNFTKPQDGDVIAPLITPQKKEWND STSVQNPTRLREASKSRVQLFKDDPM


    Database link: Q9Y3Z3

  • Positive control
    • WB: HEK-293T cell lysate transfected with pCMV6-ENTRY SAMHD1 cDNA; HAP1, K562, HepG2, HeLa, SVT2, A549, COS-7, Jurkat, MDCK, PC-12, and MCF7 cell extracts; Mouse spleen extract. IHC-P: Human kidney, kidney carcinoma, liver, ovary adenocarcinoma, pancreas, thyroid carcinoma, prostate carcinoma, bladder carcinoma and tonsil tissues. ICC/IF: COS-7 cells transiently transfected with pCMV6-ENTRY SAMHD1. Flow Cyt: HEK-293T cells transfected with pCMV6-ENTRY SAMHD1; HeLa and Jurkat cells.
  • General notes

    The clone number has been updated from 1A1 to OTI1A1, both clone numbers name the same antibody clone.

Properties

Applications

Our Abpromise guarantee covers the use of ab128107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 72 kDa.
IHC-P 1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100.
ICC/IF 1/100.

Target

  • Function
    Putative nuclease involved in innate immune response by acting as a negative regulator of the cell-intrinsic antiviral response. May play a role in mediating proinflammatory responses to TNF-alpha signaling.
  • Tissue specificity
    Expressed in heart, skeletal muscle, spleen, liver, small intestine, placenta, lung and peripheral blood leukocytes. No expression is seen in brain and thymus.
  • Involvement in disease
    Defects in SAMHD1 are the cause of Aicardi-Goutieres syndrome type 5 (AGS5) [MIM:612952]. A form of Aicardi-Goutieres syndrome, a genetically heterogeneous disease characterized by cerebral atrophy, leukoencephalopathy, intracranial calcifications, chronic cerebrospinal fluid (CSF) lymphocytosis, increased CSF alpha-interferon, and negative serologic investigations for common prenatal infection. Clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process. Severe neurological dysfunctions manifest in infancy as progressive microcephaly, spasticity, dystonic posturing and profound psychomotor retardation. Death often occurs in early childhood.
  • Sequence similarities
    Belongs to the SAMHD1 family.
    Contains 1 HD domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CHBL2 antibody
    • DCIP antibody
    • Dendritic cell derived IFNG induced protein antibody
    • Dendritic cell-derived IFNG-induced protein antibody
    • Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 antibody
    • dNTPase antibody
    • HD domain containing 1 antibody
    • HDDC1 antibody
    • Mg11 antibody
    • Monocyte protein 5 antibody
    • MOP 5 antibody
    • MOP-5 antibody
    • MOP5 antibody
    • OTTHUMP00000030889 antibody
    • SAM domain and HD domain 1 antibody
    • SAM domain and HD domain containing protein 1 antibody
    • SAM domain and HD domain-containing protein 1 antibody
    • SAMH1_HUMAN antibody
    • Samhd1 antibody
    • SBBI88 antibody
    see all

Images

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: SAMHD1 knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lanes 1 - 3: Merged signal (red and green). Green - ab128107 observed at 70 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab128107 was shown to recognize SAMHD1 when SAMHD1 knockout samples were used, along with additional cross-reactive bands. Wild-type and SAMHD1 knockout samples were subjected to SDS-PAGE. ab128107 and ab181602 (loading control to GAPDH) were diluted 1/500 and 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-SAMHD1 antibody [OTI1A1] (ab128107) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY SAMHD1 cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 72 kDa

  • All lanes : Anti-SAMHD1 antibody [OTI1A1] (ab128107) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 (human lung carcinoma cell line) cell extract
    Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) cell extract
    Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) cell extract
    Lane 7 : MDCK (Canine kidney cell line) cell extract
    Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract
    Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell extract

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 72 kDa

  • Paraffin-embedded human kidney tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.

  • Paraffin-embedded human kidney carcinoma tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.

  • Paraffin-embedded human liver tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.

  • Paraffin-embedded human ovary adenocarcinoma tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.

  • Paraffin-embedded human pancreas tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.

  • Anti-SAMHD1 antibody [OTI1A1] (ab128107) at 1/200 dilution + Mouse spleen extract at 10 µg

    Predicted band size: 72 kDa

  • Paraffin-embedded human thyroid carcinoma tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.

  • Paraffin-embedded human prostate carcinoma tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.

  • Paraffin-embedded human bladder carcinoma tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.

  • Paraffin-embedded human tonsil tissue stained for SAMHD1 using ab128107 at 1/150 dilution in immunohistochemical analysis.

  • COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY SAMHD1 cDNA  stained forSAMHD1 using ab128107 at 1/100 dilution in ICC/IF.

  • Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY SAMHD1 overexpress plasmid (Red) or empty vector control plasmid (Blue) stained for SAMHD1 using ab128107 at 1/100 dilution.

  • Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SAMHD1 using ab128107 at 1/100 dilution (Red) compared to a nonspecific negative control (Blue).

  • Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells stained for SAMHD1 using ab128107 at 1/100 dilution (Red) compared to a nonspecific negative control (Blue).

References

This product has been referenced in:
  • Tramentozzi E  et al. The dNTP triphosphohydrolase activity of SAMHD1 persists during S-phase when the enzyme is phosphorylated at T592. Cell Cycle 17:1102-1114 (2018). Read more (PubMed: 30039733) »
  • Calonge E  et al. Different Expression of Interferon-Stimulated Genes in Response to HIV-1 Infection in Dendritic Cells Based on Their Maturation State. J Virol 91:N/A (2017). Read more (PubMed: 28148784) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

The concentration of lot GR82285 is 1.17mg/ml.

Read More

Answer

Thank you for contacting us.
I am sorry to hear that the antibodies don't seem to work as expected.

As we discussed over the phone, the following protocols tips can help with improving your results:

1) loading more protein onto the gel (e.g. 20-30 ug or more if needed)
2) using more primary antibody (e.g. 1/500 per recommended dilutions on the datasheet)
3) check if the secondary antibody is working well with other primary antibodies

I hope these tips will be of help.
Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up