Product nameAnti-SAMM50 antibody [EPR8717]
See all SAMM50 primary antibodies
DescriptionRabbit monoclonal [EPR8717] to SAMM50
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide, corresponding to N terminal residues of Human SAMM50 (Uniprot: Q9Y512).
- Human fetal heart lysate; A431, HeLa and HepG2 cell lysates; Human stomach tissue; HeLa cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab167430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000 - 1/200000. Predicted molecular weight: 52 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMay be required for the assembly pathway of mitochondrial outer membrane proteins.
Sequence similaritiesBelongs to the SAM50/omp85 family.
DomainIts C-terminal part seems to contain many membrane-spanning sided beta-sheets, that have the potential to adopt a transmembrane beta-barrel type structure.
Cellular localizationMitochondrion outer membrane. Cytoplasm.
- Information by UniProt
- CGI 51 antibody
- CGI-51 antibody
- FLJ35825 antibody
All lanes : Anti-SAMM50 antibody [EPR8717] (ab167430) at 1/50000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human stomach tissue, labeling SAMM50 with ab167430 at 1/50 dilution.
Flow cytometric analysis of permeabilized HeLa cells labeling SAMM50 with ab167430 at 1/10 dilution (red) or a rabbit IgG (negative) (green).