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Rabbit monoclonal [EPR8717] to SAMM50
, Flow Cytmore detailsUnsuitable for:
ICC/IF or IP
Mouse, Rat, Human
Synthetic peptide, corresponding to N terminal residues of Human SAMM50 (Uniprot: Q9Y512).
- Human fetal heart lysate; A431, HeLa and HepG2 cell lysates; Human stomach tissue; HeLa cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50000 - 1/200000. Predicted molecular weight: 52 kDa.
||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for ICC/IF or IP.
May be required for the assembly pathway of mitochondrial outer membrane proteins.
Belongs to the SAM50/omp85 family.
Its C-terminal part seems to contain many membrane-spanning sided beta-sheets, that have the potential to adopt a transmembrane beta-barrel type structure.
Mitochondrion outer membrane. Cytoplasm.
Information by UniProt
- CGI 51 antibody
- CGI-51 antibody
- FLJ35825 antibody
Western blot - Anti-SAMM50 antibody [EPR8717] (ab167430)
All lanes : Anti-SAMM50 antibody [EPR8717] (ab167430) at 1/50000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAMM50 antibody [EPR8717] (ab167430)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human stomach tissue, labeling SAMM50 with ab167430 at 1/50 dilution.
Flow Cytometry - Anti-SAMM50 antibody [EPR8717] (ab167430)
Flow cytometric analysis of permeabilized HeLa cells labeling SAMM50 with ab167430 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"