Overview

  • Product name
    Anti-SAMM50 antibody [EPR8717]
    See all SAMM50 primary antibodies
  • Description
    Rabbit monoclonal [EPR8717] to SAMM50
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to N terminal residues of Human SAMM50 (Uniprot: Q9Y512).

  • Positive control
    • Human fetal heart lysate; A431, HeLa and HepG2 cell lysates; Human stomach tissue; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab167430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Predicted molecular weight: 52 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      May be required for the assembly pathway of mitochondrial outer membrane proteins.
    • Sequence similarities
      Belongs to the SAM50/omp85 family.
    • Domain
      Its C-terminal part seems to contain many membrane-spanning sided beta-sheets, that have the potential to adopt a transmembrane beta-barrel type structure.
    • Cellular localization
      Mitochondrion outer membrane. Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • CGI 51 antibody
      • CGI-51 antibody
      • FLJ35825 antibody
      • FLJ42905 antibody
      • FLJ99036 antibody
      • OMP85 antibody
      • SAM50 antibody
      • SAM50_HUMAN antibody
      • Samm50 antibody
      • sorting and assembly machinery 50kDa antibody
      • sorting and assembly machinery component 50 homolog (S. cerevisiae) antibody
      • Sorting and assembly machinery component 50 homolog antibody
      • sorting and assembly machinery component 50, S. cerevisiae, homolog of antibody
      • TOB55 antibody
      • Transformation-related gene 3 protein antibody
      • TRG-3 antibody
      • YNL026W antibody
      see all

    Images

    • All lanes : Anti-SAMM50 antibody [EPR8717] (ab167430) at 1/50000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : A431 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 52 kDa

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human stomach tissue, labeling SAMM50 with ab167430 at 1/50 dilution.
    • Flow cytometric analysis of permeabilized HeLa cells labeling SAMM50 with ab167430 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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