Key features and details
- Rabbit polyclonal to SAMM50/SAM50
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SAMM50/SAM50 antibody
See all SAMM50/SAM50 primary antibodies
DescriptionRabbit polyclonal to SAMM50/SAM50
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Recombinant fragment corresponding to Human SAMM50/SAM50 aa 1-220.
MGTVHARSLEPLPSSGPDFGGLGEEAEFVEVEPEAKQEILENKDVVVQHV HFDGLGRTKDDIIICEIGDVFKAKNLIEVMRKSHEAREKLLRLGIFRQVD VLIDTCQGDDALPNGLDVTFEVTELRRLTGSYNTMVGNNEGSMVLGLKLP NLLGRAEKVTFQFSYGTKETSYGLSFFKPRPGNFERNFSVNLYKVTGQFP WSSLRETDRGMSAEYSFPIW
Database link: Q9Y512
- WB: Mouse gonadal lysate; A-375, A431, HEK-293T, HeLa, A549, HepG2, U-251 MG and LO2 whole cell lysates. IHC-P: Human liver cancer and skeletal muscle tissues.
This product was previously labelled as SAMM50
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab235308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 52 kDa.|
FunctionMay be required for the assembly pathway of mitochondrial outer membrane proteins.
Sequence similaritiesBelongs to the SAM50/omp85 family.
DomainIts C-terminal part seems to contain many membrane-spanning sided beta-sheets, that have the potential to adopt a transmembrane beta-barrel type structure.
Cellular localizationMitochondrion outer membrane. Cytoplasm.
- Information by UniProt
- CGI 51 antibody
- CGI-51 antibody
- FLJ35825 antibody
All lanes : Anti-SAMM50/SAM50 antibody (ab235308) at 1/1000 dilution
Lane 1 : Mouse gonadal lysate
Lane 2 : A-375 (human malignant melanoma cell line) whole cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 6 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 7 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 8 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 9 : LO2 (human immortal liver cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 52 kDa
Paraffin-embedded human liver cancer tissue stained for SAMM50/SAM50 using ab235308 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human skeletal muscle tissue stained for SAMM50/SAM50 using ab235308 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235308 has not yet been referenced specifically in any publications.