Key features and details
- Rabbit polyclonal to SAMM50/SAM50
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SAMM50/SAM50 antibody
See all SAMM50/SAM50 primary antibodies
DescriptionRabbit polyclonal to SAMM50/SAM50
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
- WB: HL-60, NIH/3T3 and NBT-II whole cell lysates. IHC-P: Human parathyroid gland, pancreas, small intestine, testis and kidney tissue. ICC/IF: A431 cells.
This product was previously labelled as SAMM50
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab246987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 52 kDa.|
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
FunctionMay be required for the assembly pathway of mitochondrial outer membrane proteins.
Sequence similaritiesBelongs to the SAM50/omp85 family.
DomainIts C-terminal part seems to contain many membrane-spanning sided beta-sheets, that have the potential to adopt a transmembrane beta-barrel type structure.
Cellular localizationMitochondrion outer membrane. Cytoplasm.
- Information by UniProt
- CGI 51 antibody
- CGI-51 antibody
- FLJ35825 antibody
Formalin-fixed, paraffin-embedded human pancreas tissue stained for SAMM50 with ab246987 at a 1/1000 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human kidney tissue stained for SAMM50 with ab246987 at a 1/1000 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human testis tissue stained for SAMM50 with ab246987 at a 1/1000 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human small intestine tissue stained for SAMM50 with ab246987 at a 1/1000 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human parathyroid gland tissue stained for SAMM50 with ab246987 at a 1/1000 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SAMM50/SAM50 (green) using ab246987 at 4 μg/ml in ICC/IF.
All lanes : Anti-SAMM50/SAM50 antibody (ab246987) at 0.4 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : NBT-II (rat Wistar bladder tumor cell line) whole cell lysate
Predicted band size: 52 kDa
Anti-SAMM50/SAM50 antibody (ab246987) at 0.4 µg + HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Predicted band size: 52 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246987 has not yet been referenced specifically in any publications.