Anti-SAMM50/SAM50 antibody (ab246987)
Key features and details
- Rabbit polyclonal to SAMM50/SAM50
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SAMM50/SAM50 antibody
See all SAMM50/SAM50 primary antibodies -
Description
Rabbit polyclonal to SAMM50/SAM50 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to Human SAMM50/SAM50 aa 1-150.
Database link: Q9Y512 -
Positive control
- WB: HL-60, NIH/3T3 and NBT-II whole cell lysates. IHC-P: Human parathyroid gland, pancreas, small intestine, testis and kidney tissue. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab246987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 52 kDa.
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 52 kDa. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Function
May be required for the assembly pathway of mitochondrial outer membrane proteins. -
Sequence similarities
Belongs to the SAM50/omp85 family. -
Domain
Its C-terminal part seems to contain many membrane-spanning sided beta-sheets, that have the potential to adopt a transmembrane beta-barrel type structure. -
Cellular localization
Mitochondrion outer membrane. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 618777 Cow
- Entrez Gene: 25813 Human
- Entrez Gene: 68653 Mouse
- Entrez Gene: 300111 Rat
- Omim: 612058 Human
- SwissProt: Q2HJ55 Cow
- SwissProt: Q9Y512 Human
- SwissProt: Q8BGH2 Mouse
see all -
Alternative names
- CGI 51 antibody
- CGI-51 antibody
- FLJ35825 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human pancreas tissue stained for SAMM50 with ab246987 at a 1/1000 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for SAMM50 with ab246987 at a 1/1000 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human testis tissue stained for SAMM50 with ab246987 at a 1/1000 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human small intestine tissue stained for SAMM50 with ab246987 at a 1/1000 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human parathyroid gland tissue stained for SAMM50 with ab246987 at a 1/1000 dilution in immunohistochemical analysis.
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PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SAMM50/SAM50 (green) using ab246987 at 4 μg/ml in ICC/IF.
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All lanes : Anti-SAMM50/SAM50 antibody (ab246987) at 0.4 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : NBT-II (rat Wistar bladder tumor cell line) whole cell lysate
Predicted band size: 52 kDa -
Anti-SAMM50/SAM50 antibody (ab246987) at 0.4 µg + HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Predicted band size: 52 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab246987 has been referenced in 1 publication.
- Lu X et al. Ultrastructural and proteomic profiling of mitochondria-associated endoplasmic reticulum membranes reveal aging signatures in striated muscle. Cell Death Dis 13:296 (2022). PubMed: 35368021