Recombinant
RabMAb

Recombinant Anti-SAMM50/SAM50 antibody [EPR8718] (ab133709)

Overview

  • Product name

    Anti-SAMM50/SAM50 antibody [EPR8718]
    See all SAMM50/SAM50 primary antibodies
  • Description

    Rabbit monoclonal [EPR8718] to SAMM50/SAM50
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human SAMM50/SAM50 aa 350-450.
    Database link: Q9Y512

  • Positive control

    • WB: Human fetal heart lysate, A431 cell lysate, HeLa cell lysate, HepG2 cell lysate IHC-P: Human kidney tissue, Human ovarian carcinoma tissue
  • General notes

     

     

     This product was previously labelled as SAMM50

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IHC-P 1/50 - 1/100. Perform antigen retrieval before commencing with IHC staining protocol
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function

      May be required for the assembly pathway of mitochondrial outer membrane proteins.
    • Sequence similarities

      Belongs to the SAM50/omp85 family.
    • Domain

      Its C-terminal part seems to contain many membrane-spanning sided beta-sheets, that have the potential to adopt a transmembrane beta-barrel type structure.
    • Cellular localization

      Mitochondrion outer membrane. Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • CGI 51 antibody
      • CGI-51 antibody
      • FLJ35825 antibody
      • FLJ42905 antibody
      • FLJ99036 antibody
      • OMP85 antibody
      • SAM50 antibody
      • SAM50_HUMAN antibody
      • Samm50 antibody
      • sorting and assembly machinery 50kDa antibody
      • sorting and assembly machinery component 50 homolog (S. cerevisiae) antibody
      • Sorting and assembly machinery component 50 homolog antibody
      • sorting and assembly machinery component 50, S. cerevisiae, homolog of antibody
      • TOB55 antibody
      • Transformation-related gene 3 protein antibody
      • TRG-3 antibody
      • YNL026W antibody
      see all

    Images

    • All lanes : Anti-SAMM50/SAM50 antibody [EPR8718] (ab133709) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate

      Lane 2 : A431 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 52 kDa

    • ab133709, at 1/50 dilution, staining SAMM50/SAM50 in paraffin embedded Human kidney tissue using immunohistochemical analysis.

    • ab133709, at 1/50 dilution, staining SAMM50/SAM50 in paraffin embedded Human ovarian carcinoma tissue using immunohistochemical analysis.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Le Guerroué F  et al. Autophagosomal Content Profiling Reveals an LC3C-Dependent Piecemeal Mitophagy Pathway. Mol Cell 68:786-796.e6 (2017). Read more (PubMed: 29149599) »
    • Ding C  et al. Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure. Sci Rep 5:16064 (2015). Read more (PubMed: 26530328) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (Mouse brain homogenate, and mitochondrial fraction)
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Loading amount
    20 µg
    Specification
    Mouse brain homogenate, and mitochondrial fraction
    Blocking step
    SEA Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C

    Svitlana Yablonska

    Verified customer

    Submitted Jun 28 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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