Product nameAnti-SAMM50/SAM50 antibody [EPR8718]
See all SAMM50/SAM50 primary antibodies
DescriptionRabbit monoclonal [EPR8718] to SAMM50/SAM50
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Mouse, Rat, Human
corresponding to Human SAMM50/SAM50 aa 350-450.
Database link: Q9Y512
- WB: Human fetal heart lysate, A431 cell lysate, HeLa cell lysate, HepG2 cell lysate IHC-P: Human kidney tissue, Human ovarian carcinoma tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.30 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).|
|IHC-P||1/50 - 1/100. Perform antigen retrieval before commencing with IHC staining protocol|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMay be required for the assembly pathway of mitochondrial outer membrane proteins.
Sequence similaritiesBelongs to the SAM50/omp85 family.
DomainIts C-terminal part seems to contain many membrane-spanning sided beta-sheets, that have the potential to adopt a transmembrane beta-barrel type structure.
Cellular localizationMitochondrion outer membrane. Cytoplasm.
- Information by UniProt
- CGI 51 antibody
- CGI-51 antibody
- FLJ35825 antibody
All lanes : Anti-SAMM50/SAM50 antibody [EPR8718] (ab133709) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 52 kDa
ab133709, at 1/50 dilution, staining SAMM50/SAM50 in paraffin embedded Human kidney tissue using immunohistochemical analysis.
ab133709, at 1/50 dilution, staining SAMM50/SAM50 in paraffin embedded Human ovarian carcinoma tissue using immunohistochemical analysis.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Le Guerroué F et al. Autophagosomal Content Profiling Reveals an LC3C-Dependent Piecemeal Mitophagy Pathway. Mol Cell 68:786-796.e6 (2017). Read more (PubMed: 29149599) »
- Ding C et al. Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure. Sci Rep 5:16064 (2015). Read more (PubMed: 26530328) »