Key features and details
- Mouse monoclonal [OTI7F5] to SAMSN1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-SAMSN1 antibody [OTI7F5]
See all SAMSN1 primary antibodies
DescriptionMouse monoclonal [OTI7F5] to SAMSN1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human SAMSN1 aa 1-250. (NP_071419) produced in E.coli.
MLKRKPSNVSEKEKHQKPKRSSSFGNFDRFRNNSLSKPDDSTEAHEGDPT NGSGEQSKTSNNGGGLGKKMRAISWTMKKKVGKKYIKALSEEKDEEDGEN AHPYRNSDPVIGTHTEKVSLKASDSMDSLYSGQSSSSGITSCSDGTSNRD SFRLDDDGPYSGPFCGRARVHTDFTPSPYDTDSLKIKKGDIIDIICKTPM GMWTGMLNNKVGNFKFIYVDVISEEEAAPKKIKANRRSNSKKSKTLQEFL
Database link: Q9NSI8
- WB: HEK-293T transfected with pCMV6-ENTRY SAMSN1, whole cell lysate. IHC-P: Human lymphoma and tonsil tissue.
Clone OTI7F5 (formerly 7F5).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes.
|WB||1/2000. Predicted molecular weight: 42 kDa.|
FunctionNegative regulator of B-cell activation. Down-regulates cell proliferation (in vitro). Promotes RAC1-dependent membrane ruffle formation and reorganization of the actin cytoskeleton. Regulates cell spreading and cell polarization. Stimulates HDAC1 activity. Regulates LYN activity by modulating its tyrosine phosphorylation.
Tissue specificityDetected in peripheral blood B-cells (at protein level). Detected in spleen, liver and peripheral blood.
Sequence similaritiesContains 1 SAM (sterile alpha motif) domain.
Contains 1 SH3 domain.
Cellular localizationNucleus. Cytoplasm. Cell projection > ruffle. Shuttles between cytoplasm and nucleus. Colocalizes with the actin cytoskeleton and actin-rich membrane ruffles.
- Information by UniProt
- HACS1 antibody
- Hematopoietic adapter containing SH3 and sterile α motif (SAM) domains 1 antibody
- Hematopoietic adapter containing SH3 and sterile alpha motif (SAM) domains 1 antibody
All lanes : Anti-SAMSN1 antibody [OTI7F5] (ab236410) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control, whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY SAMSN1, whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 42 kDa
Paraffin-embedded human lymphoma tissue stained for SAMSN1 using ab236410 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human tonsil tissue stained for SAMSN1 using ab236410 at 1/150 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236410 has not yet been referenced specifically in any publications.