Overview

  • Product name

  • Description

    Rabbit polyclonal to SAP130
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    A synthetic peptide corresponding to a region between amino acids 998-1048 of Human SAP130 (NP_078821.2).

  • Positive control

    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab114978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Acts as a transcriptional repressor. May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.
    • Tissue specificity

      Expressed in various cancer cell ines.
    • Sequence similarities

      Belongs to the SAP130 family.
    • Domain

      The N-terminus may interact with a transcriptional coactivator.
      The C-terminus may interact with HDAC-dependent and HDAC-independent corepressors.
    • Post-translational
      modifications

      Acetylated.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 130 kDa Sin3-associated polypeptide antibody
      • FLJ12761 antibody
      • Histone deacetylase complex subunit SAP130 antibody
      • KIAA0017 antibody
      • mSin3A associated protein 130 antibody
      • Pre mRNA splicing factor SF3b 130 kDa subunit antibody
      • RSE1 antibody
      • SAP 130 antibody
      • Sap130 antibody
      • SF3b 130 antibody
      • SF3b130 antibody
      • SF3B3 antibody
      • Sin3-associated polypeptide p130 antibody
      • Sin3A associated protein 130kDa antibody
      • Sin3A associated protein antibody
      • SP130_HUMAN antibody
      • Spliceosome associated protein 130 antibody
      • Splicing factor 3b subunit 3 130kDa antibody
      • Splicing factor 3b subunit 3 antibody
      • STAF 130 antibody
      • STAF130 antibody
      see all

    Images

    • ab114978, used for IP at 3 µg/mg lysate,staining SAP130 in HeLa whole cell lysate (1 µg for IP, 20% of IP loaded).

      Detection: Chemiluminescence with an exposure time of 10 seconds.

    References

    This product has been referenced in:

    • Li W  et al. The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer. J Clin Invest 127:3421-3440 (2017). Read more (PubMed: 28805661) »
    • Salas-Armenteros I  et al. Human THO-Sin3A interaction reveals new mechanisms to prevent R-loops that cause genome instability. EMBO J 36:3532-3547 (2017). Read more (PubMed: 29074626) »
    See all 2 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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