Key features and details
- Rabbit polyclonal to SAP18
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SAP18 antibody
See all SAP18 primary antibodies
DescriptionRabbit polyclonal to SAP18
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Zebrafish, Rhesus monkey
Recombinant fragment within Human SAP18 (internal sequence). The exact sequence is proprietary.
Database link: O00422
- WB: K562 whole cell and nuclear extracts.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 18 kDa.|
FunctionComponent of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism.
Sequence similaritiesBelongs to the SAP18 family.
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.
- Information by UniProt
- 18 kDa Sin3-associated polypeptide antibody
- 2HOR0202 antibody
- Cell growth-inhibiting gene 38 protein antibody
All lanes : Anti-SAP18 antibody (ab226757) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell extract
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) nuclear extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 18 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226757 has not yet been referenced specifically in any publications.