• Product name
  • Description
    Rabbit polyclonal to SAP18
  • Host species
  • Tested applications
    Suitable for: IP, ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SAP18.

    Read Abcam's proprietary immunogen policy (Peptide available as ab30465.)

  • Positive control
    • Recombinant Human SAP18 protein (ab86702) can be used as a positive control in WB. HeLa (Human epithelial carcinoma cell line) Nuclear Lysate.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab31748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ICC/IF: Use at a concentration of 1 µg/ml.
    IHC-P: 1/650. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
    IP: Use at a concentration of 1.6 µg/ml (PMID 19503603).
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 18 kDa). Detection of the band is blocked by addition of the immunizing peptide ab30465.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism.
    • Tissue specificity
    • Sequence similarities
      Belongs to the SAP18 family.
    • Cellular localization
      Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 18 kDa Sin3-associated polypeptide antibody
      • 2HOR0202 antibody
      • Cell growth-inhibiting gene 38 protein antibody
      • Histone deacetylase complex subunit SAP18 antibody
      • SAP18 antibody
      • SAP18_HUMAN antibody
      • Sin3-associated polypeptide p18 antibody
      see all


    • All lanes : Anti-SAP18 antibody (ab31748) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate with Human SAP18 peptide (ab30465) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size: 18 kDa
      Observed band size: 21 kDa
      why is the actual band size different from the predicted?

    • ICC/IF image of ab31748 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31748, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
    • Image courtesy of Human Protein Atlas
      ab31748 stainning SAP18 in paraffin-embedded human cervix, showing a distinct nuclear staining of the surface epithelial (squamous) cells. Tissue sections (4µm) were incubated with ab31748 (1/650 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab31748 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org


    This product has been referenced in:
    • Zhu J  et al. NKX2-8 deletion-induced reprogramming of fatty acid metabolism confers chemoresistance in epithelial ovarian cancer. EBioMedicine 43:238-252 (2019). Read more (PubMed: 31047858) »
    • Sikorski K  et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). Read more (PubMed: 30377371) »
    See all 4 Publications for this product

    Customer reviews and Q&As


    Thank you for your inquiry. This antibody is indeed expected to cross react to the following species: Mouse, Rat, Cow, Xenopus laevis and Zebrafish. But this has not yet been experimentally tested. Unfortunately, this antibody's immunogen only shares 2% homology with the sequence for SAP18 from A.thaliana. The antibody therefore will not cross-react with A.thaliana. Please use the following link to review all antibodies for A.thaliana that we offer at the moment: https://www.abcam.com/index.html?pageconfig=resource&rid=11670&viapagetrap=arabidopsis I am sorry I could not give a positive answer on this occasion and hope this information is nevertheless helpful.

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