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Human Cell lysate - whole cell (HeLa)
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
16 hour(s) and 0 minute(s)
Non-Abcam antibody was used: anti-Rabbit
Host species: Goat
Conjugation: Horse Radish Peroxidase
2 minute(s) and 0 second(s)
Specific: 18 kDa Non-specific: 30 kDa
Immunoprecipitation with Acinus, a protein known to interact with SAP18 (lane 6). Only the 18 kDa band is observed, the unspecific band that was detected in lysates is lost.
Immunoprecipitation with FLAG-peptide (no binding partner). No bands were detected (lane 4).
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Submitted May 01 2007