Western blot abreview for Anti-SAP18 antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
50 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 18 kDa Non-specific: 30 kDa
Positive control
Immunoprecipitation with Acinus, a protein known to interact with SAP18 (lane 6). Only the 18 kDa band is observed, the unspecific band that was detected in lysates is lost.
Negative control
Immunoprecipitation with FLAG-peptide (no binding partner). No bands were detected (lane 4).

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Submitted May 01 2007

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