Recombinant Anti-SAP18 antibody [EPR12956(B)] (ab175920)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12956(B)] to SAP18
- Suitable for: WB, ICC/IF, IP
- Reacts with: Rat, Human
Overview
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Product name
Anti-SAP18 antibody [EPR12956(B)]
See all SAP18 primary antibodies -
Description
Rabbit monoclonal [EPR12956(B)] to SAP18 -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanIP HumanWB Human -
Immunogen
Synthetic peptide within Human SAP18 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: O00422 -
Positive control
- Jurkat, K562, 293T and PC-12 cell lysates. 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2 -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12956(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC/IF |
Human
|
IP |
Human
|
WB |
Human
|
All applications |
Mouse
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
|
|
ICC/IF |
1/10 - 1/100.
|
|
IP |
1/10 - 1/100.
|
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 18 kDa. |
ICC/IF
1/10 - 1/100. |
IP
1/10 - 1/100. |
Target
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Function
Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the SAP18 family. -
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10284 Human
- Entrez Gene: 20220 Mouse
- Entrez Gene: 290284 Rat
- Omim: 602949 Human
- SwissProt: O00422 Human
- SwissProt: O55128 Mouse
- Unigene: 524899 Human
- Unigene: 30173 Mouse
see all -
Alternative names
- 18 kDa Sin3-associated polypeptide antibody
- 2HOR0202 antibody
- Cell growth-inhibiting gene 38 protein antibody
see all
Images
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All lanes : Anti-SAP18 antibody [EPR12956(B)] (ab175920) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Hela cell lysate
Lane 4 : 293T cell lysate
Lane 5 : PC-12 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 18 kDa -
Immunofluorescent staining of 293T cells labeling SAP18 with ab175920 at 1/100 dilution.
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Western blot analysis on immunoprecipitation pellet from K562 cell lysate labeling SAP18 with ab175920 at 1/10 dilution
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab175920 has not yet been referenced specifically in any publications.