Recombinant
RabMAb

Recombinant Anti-SAP18 antibody [EPR12956(B)] (ab175920)

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Overview

  • Product name

    Anti-SAP18 antibody [EPR12956(B)]
    See all SAP18 primary antibodies

  • Description

    Rabbit monoclonal [EPR12956(B)] to SAP18

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SAP18 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O00422

  • Positive control

    • Jurkat, K562, 293T and PC-12 cell lysates. 293T cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12956(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
ICC/IF 1/10 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function

      Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism.

    • Tissue specificity

      Ubiquitous.

    • Sequence similarities

      Belongs to the SAP18 family.

    • Cellular localization

      Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 18 kDa Sin3-associated polypeptide antibody
      • 2HOR0202 antibody
      • Cell growth-inhibiting gene 38 protein antibody
      • Histone deacetylase complex subunit SAP18 antibody
      • SAP18 antibody
      • SAP18_HUMAN antibody
      • Sin3-associated polypeptide p18 antibody
      see all

    Images

    • Western blot - Anti-SAP18 antibody [EPR12956(B)] (ab175920)
      Western blot - Anti-SAP18 antibody [EPR12956(B)] (ab175920)
      All lanes : Anti-SAP18 antibody [EPR12956(B)] (ab175920) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Hela cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : PC-12 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 18 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-SAP18 antibody [EPR12956(B)] (ab175920)
      Immunocytochemistry/ Immunofluorescence - Anti-SAP18 antibody [EPR12956(B)] (ab175920)

      Immunofluorescent staining of 293T cells labeling SAP18 with ab175920 at 1/100 dilution.

    • Immunoprecipitation - Anti-SAP18 antibody [EPR12956(B)] (ab175920)
      Immunoprecipitation - Anti-SAP18 antibody [EPR12956(B)] (ab175920)

      Western blot analysis on immunoprecipitation pellet from K562 cell lysate labeling SAP18 with ab175920 at 1/10 dilution

    References

    ab175920 has not yet been referenced specifically in any publications.

    Publishing research using ab175920? Please let us know so that we can cite the reference in this datasheet.

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