RecombinantRabMAb

Recombinant Anti-SAP18 antibody [EPR12956(B)] (ab175920)

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Western blot - Anti-SAP18 antibody [EPR12956(B)] (ab175920)
  • Immunocytochemistry/ Immunofluorescence - Anti-SAP18 antibody [EPR12956(B)] (ab175920)
  • Immunoprecipitation - Anti-SAP18 antibody [EPR12956(B)] (ab175920)
  • Anti-SAP18 antibody [EPR12956(B)] (ab175920)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12956(B)] to SAP18
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-SAP18 antibody [EPR12956(B)]
    See all SAP18 primary antibodies

  • Description

    Rabbit monoclonal [EPR12956(B)] to SAP18

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SAP18 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O00422

  • Positive control

    • Jurkat, K562, 293T and PC-12 cell lysates. 293T cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12956(B)

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IP
Human
WB
Human
All applications
Mouse
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
ICC/IF
1/10 - 1/100.
IP
1/10 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
ICC/IF
1/10 - 1/100.
IP
1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function

      Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism.

    • Tissue specificity

      Ubiquitous.

    • Sequence similarities

      Belongs to the SAP18 family.

    • Cellular localization

      Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 18 kDa Sin3-associated polypeptide antibody
      • 2HOR0202 antibody
      • Cell growth-inhibiting gene 38 protein antibody
      • Histone deacetylase complex subunit SAP18 antibody
      • SAP18 antibody
      • SAP18_HUMAN antibody
      • Sin3-associated polypeptide p18 antibody
      see all

    Images

    • Western blot - Anti-SAP18 antibody [EPR12956(B)] (ab175920)
      Western blot - Anti-SAP18 antibody [EPR12956(B)] (ab175920)
      All lanes : Anti-SAP18 antibody [EPR12956(B)] (ab175920) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Hela cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : PC-12 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 18 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-SAP18 antibody [EPR12956(B)] (ab175920)
      Immunocytochemistry/ Immunofluorescence - Anti-SAP18 antibody [EPR12956(B)] (ab175920)

      Immunofluorescent staining of 293T cells labeling SAP18 with ab175920 at 1/100 dilution.

    • Immunoprecipitation - Anti-SAP18 antibody [EPR12956(B)] (ab175920)
      Immunoprecipitation - Anti-SAP18 antibody [EPR12956(B)] (ab175920)

      Western blot analysis on immunoprecipitation pellet from K562 cell lysate labeling SAP18 with ab175920 at 1/10 dilution

    • Anti-SAP18 antibody [EPR12956(B)] (ab175920)
      Anti-SAP18 antibody [EPR12956(B)] (ab175920)

    References (0)

    Publishing research using ab175920? Please let us know so that we can cite the reference in this datasheet.

    ab175920 has not yet been referenced specifically in any publications.

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