Product nameAnti-SAP18 antibody [EPR12956(B)]
See all SAP18 primary antibodies
DescriptionRabbit monoclonal [EPR12956(B)] to SAP18
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human SAP18 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: O00422
- Jurkat, K562, 293T and PC-12 cell lysates. 293T cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175920 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 18 kDa.|
|ICC/IF||1/10 - 1/100.|
|IP||1/10 - 1/100.|
FunctionComponent of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism.
Sequence similaritiesBelongs to the SAP18 family.
Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm.
- Information by UniProt
- 18 kDa Sin3-associated polypeptide antibody
- 2HOR0202 antibody
- Cell growth-inhibiting gene 38 protein antibody
All lanes : Anti-SAP18 antibody [EPR12956(B)] (ab175920) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Hela cell lysate
Lane 4 : 293T cell lysate
Lane 5 : PC-12 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 18 kDa
Immunofluorescent staining of 293T cells labeling SAP18 with ab175920 at 1/100 dilution.
Western blot analysis on immunoprecipitation pellet from K562 cell lysate labeling SAP18 with ab175920 at 1/10 dilution
ab175920 has not yet been referenced specifically in any publications.