Key features and details
- Rabbit polyclonal to SAP30
- Suitable for: ChIP-sequencing, WB, ChIP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SAP30 antibody
See all SAP30 primary antibodies
DescriptionRabbit polyclonal to SAP30
Tested applicationsSuitable for: ChIP-sequencing, WB, ChIPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant full length protein (His-tag) corresponding to Human SAP30 aa 1-220.
MNGFTPDEMSRGGDAAAAVAAVVAAAAAAASAGNGTGAGTGAEVPGAGAV SAAGPPGAAGPGPGQLCCLREDGERCGRAAGNASFSKRIQKSISQKKVKI ELDKSARHLYICDYHKNLIQSVRNRRKRKGSDDDGGDSPVQDIDTPEVDL YQLQVNTLRRYKRHFKLPTRPGLNKAQLVEIVGCHFRSIPVNEKDTLTYF IYSVKNDKNKSDLKVDSGVH
Database link: O75446
- WB: Nuclear extracts from HeLa cells. ChIP: HeLa cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.05% Sodium azide, 0.05% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab231804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP-sequencing||Use at an assay dependent concentration.
Chromatin from 4 million cells using 5 μg of antibody.
|ChIP||Use at an assay dependent concentration.
2 - 5 µg/ChIP reaction.
FunctionInvolved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N-CoR. Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating nucleosomal histones.
Tissue specificityExpressed in all tissues tested with highest levels in pancreas, ovary, PBL, spleen and thymus; lowest levels in brain, placenta, lung and kidney.
Sequence similaritiesBelongs to the SAP30 family.
- Information by UniProt
- 30 kDa Sin3-associated polypeptide antibody
- Histone deacetylase complex subunit SAP 30 antibody
- Histone deacetylase complex subunit SAP30 antibody
Anti-SAP30 antibody (ab231804) at 1/1000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear extracts at 20 µg
Dilution buffer: TBS-Tween containing 5% skimmed milk.
ChIP assays were performed using HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, ab231804 and optimized primer sets for qPCR. ChIP was performed using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 µg per ChIP experiment was analysed. IgG (1 µg/ IP) was used as negative IP control. QPCR was performed with primers for the EIF2S3 and BRCA1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Image shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP was performed on sheared chromatin from 4 million HeLa (Human epithelial cell line from cervix adenocarcinoma) cells using 5 µg ab231804. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Image shows the enrichment along the complete sequence and a 1.5 Mb region of human chromosome 1 (A and B) and in two genomic regions surrounding the BRCA1 and EIF2S3 genes on chromosome 17 and X, respectively (C and D).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231804 has not yet been referenced specifically in any publications.