Overview

  • Product name

    Anti-SAP97 antibody [EPR6821]
    See all SAP97 primary antibodies
  • Description

    Rabbit monoclonal [EPR6821] to SAP97
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SAP97 (N terminal). The exact sequence is proprietary.

  • Positive control

    • SH-SY5Y, HeLa and 293T cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 100 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel.
    • Tissue specificity

      Abundantly expressed in atrial myocardium (at protein level). Expressed in lung fibroblasts, cervical epithelial and B-cells (at protein level). Widely expressed, with isoforms displaying different expression profiles.
    • Sequence similarities

      Belongs to the MAGUK family.
      Contains 1 guanylate kinase-like domain.
      Contains 1 L27 domain.
      Contains 3 PDZ (DHR) domains.
      Contains 1 SH3 domain.
    • Domain

      The alternatively spliced domain I3 corresponding to amino acids (636-669) of isoform 4 is an EPB41 binding site mediating association to membranes in polarized and non-polarized cells.
      The PDZ domains may also mediate association to membranes by binding to EPB41 and ADGRA2 together with the L27 domain that binds CASK and DLG2.
      The L27 domain may regulate DLG1 self-association. The N-terminal alternatively spliced region is capable of binding several SH3 domains and also moderates the level of protein oligomerization.
    • Post-translational
      modifications

      Phosphorylated by MAPK12. Phosphorylation of Ser-232 regulates association with GRIN2A.
    • Cellular localization

      Membrane. Basolateral cell membrane. Endoplasmic reticulum membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Cell membrane, sarcolemma. Colocalizes with EPB41 at regions of intercellular contacts. Basolateral in epithelial cells. May also associate with endoplasmic reticulum membranes. Mainly found in neurons soma, moderately found at postsynaptic densities (By similarity).
    • Information by UniProt
    • Database links

    • Alternative names

      • Discs large homolog 1 antibody
      • discs large, Drosophila, homolog of, 1 antibody
      • discs, large homolog 1 (Drosophila) antibody
      • Disks large homolog 1 antibody
      • dJ1061C18.1.1 antibody
      • DKFZp761P0818 antibody
      • DKFZp781B0426 antibody
      • DLG 1 antibody
      • DLG1 antibody
      • DLG1_HUMAN antibody
      • DLGH 1 antibody
      • DLGH1 antibody
      • hDlg antibody
      • Presynaptic protein SAP97 antibody
      • SAP 97 antibody
      • SAP-97 antibody
      • SAP97 antibody
      • Synapse associated protein 97 antibody
      • Synapse-associated protein 97 antibody
      see all

    Images

    • All lanes : Anti-SAP97 antibody [EPR6821] (ab134156) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 100 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab134156 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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