Key features and details
- Rabbit polyclonal to Saporin
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-Saporin antibody
DescriptionRabbit polyclonal to Saporin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Species independent
Full length protein corresponding to Soapwort Saporin.
Database link: P20656
- WB: Purified saporin protein.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Concentration information loading...
Our Abpromise guarantee covers the use of ab228503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/2000. Predicted molecular weight: 34 kDa.|
RelevanceSaporin is obtained from the seeds of the Soapwort plant (Saponaria officinalis), a plant that grows wildly in Britain and other parts of Europe. Saporin is a plant enzyme with N-glycosidase activity that depurinates a specific nucleotide in the ribosomal RNA 28S, thus irreversibly blocking protein synthesis. It belongs to the well-characterized family of ribosome-inactivating proteins (RIPs). There are two types of RIPs: type I, which are much less cytotoxic due to the lack of the B chain and type II, which are distinguished from type I RIPs by the presence of the B chain and their ability to enter cells on their own. However, type I RIPs can still be internalized by fluid-phase endocytosis. In the case of saporin, it was reported that saporin first binds to the alpha 2 macroglobulin receptor on human cells and is then internalized to the cytosol. Upon internalization, the ribosomes are inactivated, resulting in cell death. Among all type I RIPs saporin SO6 is the most widely used because it is easily obtainable and very stable.
- Saporin antibody
All lanes : Anti-Saporin antibody (ab228503) at 1/200 dilution
Lane 1 : Rat spinal cord homogenate at 1/500 dilution
Lane 2 : Purified saporin protein at 1/3000 dilution
Lane 3 : Purified saporin protein at 1/2000 dilution
Lane 4 : Purified saporin protein at 1/500 dilution
All lanes : HRP-labeled sheep anti-rabbit
Predicted band size: 34 kDa
Observed band size: 29.5 kDa why is the actual band size different from the predicted?
Visualization: DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228503 has not yet been referenced specifically in any publications.