Product nameAnti-Saporin antibody
See all Saporin primary antibodies
DescriptionRabbit polyclonal to Saporin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Species independent
Full length protein corresponding to Soapwort Saporin.
Database link: P20656
- WB: Purified saporin protein.
FormLyophilised:Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at -20ºC for a higher stability, and at 4ºC with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
Our Abpromise guarantee covers the use of ab228503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/2000. Predicted molecular weight: 34 kDa.|
RelevanceSaporin is obtained from the seeds of the Soapwort plant (Saponaria officinalis), a plant that grows wildly in Britain and other parts of Europe. Saporin is a plant enzyme with N-glycosidase activity that depurinates a specific nucleotide in the ribosomal RNA 28S, thus irreversibly blocking protein synthesis. It belongs to the well-characterized family of ribosome-inactivating proteins (RIPs). There are two types of RIPs: type I, which are much less cytotoxic due to the lack of the B chain and type II, which are distinguished from type I RIPs by the presence of the B chain and their ability to enter cells on their own. However, type I RIPs can still be internalized by fluid-phase endocytosis. In the case of saporin, it was reported that saporin first binds to the alpha 2 macroglobulin receptor on human cells and is then internalized to the cytosol. Upon internalization, the ribosomes are inactivated, resulting in cell death. Among all type I RIPs saporin SO6 is the most widely used because it is easily obtainable and very stable.
- Saporin antibody
All lanes : Anti-Saporin antibody (ab228503) at 1/200 dilution
Lane 1 : Rat spinal cord homogenate at 1/500 dilution
Lane 2 : Purified saporin protein at 1/3000 dilution
Lane 3 : Purified saporin protein at 1/2000 dilution
Lane 4 : Purified saporin protein at 1/500 dilution
All lanes : HRP-labeled sheep anti-rabbit
Predicted band size: 34 kDa
Observed band size: 29.5 kDa why is the actual band size different from the predicted?
Visualization: DAB staining.
ab228503 has not yet been referenced specifically in any publications.